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dc.contributor.authorImanishi, Mikija
dc.contributor.authorNakamura, Atsushija
dc.contributor.authorMorisaki, Tatsuyaja
dc.contributor.authorFutaki, Shirohja
dc.contributor.alternative今西, 未来ja
dc.date.accessioned2011-02-03T00:03:04Z-
dc.date.available2011-02-03T00:03:04Z-
dc.date.issued2009-09-25ja
dc.identifier.issn0006-291Xja
dc.identifier.urihttp://hdl.handle.net/2433/134788-
dc.description.abstractOne simple and widespread method to create engineered zinc fingers targeting the desired DNA sequences is to modularly assemble multiple finger modules pre-selected to recognize each DNA triplet. However, it has become known that a sufficient DNA binding affinity is not always obtained. In order to create successful zinc finger proteins, it is important to understand the context-dependent contribution of each finger module to the DNA binding ability of the assembled zinc finger proteins. Here, we have created finger-deletion mutants of zinc finger proteins and examined the DNA bindings of these zinc fingers to clarify the contributions of each finger module. Our results indicate that not only a positive cooperativity but also a context-dependent reduction in the DNA binding activity can be induced by assembling zinc finger modules.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherElsevierja
dc.rights© 2009 Elsevier Inc.ja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectEngineered zinc finger proteinja
dc.subjectDNA bindingja
dc.subjectModular assemblyja
dc.subject.meshAnimalsja
dc.subject.meshBase Sequenceja
dc.subject.meshDNA/geneticsja
dc.subject.meshDNA/metabolismja
dc.subject.meshDNA-Binding Proteins/geneticsja
dc.subject.meshDNA-Binding Proteins/metabolismja
dc.subject.meshE-Box Elementsja
dc.subject.meshIntracellular Signaling Peptides and Proteins/geneticsja
dc.subject.meshMiceja
dc.subject.meshPeriod Circadian Proteinsja
dc.subject.meshPromoter Regions, Geneticja
dc.subject.meshSequence Deletionja
dc.subject.meshTranscription, Geneticja
dc.subject.meshZinc Fingers/geneticsja
dc.subject.meshZinc Fingers/physiologyja
dc.titlePositive and negative cooperativity of modularly assembled zinc fingers.ja
dc.type.niitypeJournal Articleja
dc.identifier.ncidAA00564395ja
dc.identifier.jtitleBiochemical and biophysical research communicationsja
dc.identifier.volume387ja
dc.identifier.issue3ja
dc.identifier.spage440ja
dc.identifier.epage443ja
dc.relation.doi10.1016/j.bbrc.2009.07.059ja
dc.textversionauthorja
dc.identifier.pmid19615972ja
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