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j.bbrc.2009.07.059.pdf | 294 kB | Adobe PDF | 見る/開く |
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DCフィールド | 値 | 言語 |
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dc.contributor.author | Imanishi, Miki | en |
dc.contributor.author | Nakamura, Atsushi | en |
dc.contributor.author | Morisaki, Tatsuya | en |
dc.contributor.author | Futaki, Shiroh | en |
dc.contributor.alternative | 今西, 未来 | ja |
dc.date.accessioned | 2011-02-03T00:03:04Z | - |
dc.date.available | 2011-02-03T00:03:04Z | - |
dc.date.issued | 2009-09-25 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/2433/134788 | - |
dc.description.abstract | One simple and widespread method to create engineered zinc fingers targeting the desired DNA sequences is to modularly assemble multiple finger modules pre-selected to recognize each DNA triplet. However, it has become known that a sufficient DNA binding affinity is not always obtained. In order to create successful zinc finger proteins, it is important to understand the context-dependent contribution of each finger module to the DNA binding ability of the assembled zinc finger proteins. Here, we have created finger-deletion mutants of zinc finger proteins and examined the DNA bindings of these zinc fingers to clarify the contributions of each finger module. Our results indicate that not only a positive cooperativity but also a context-dependent reduction in the DNA binding activity can be induced by assembling zinc finger modules. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | en |
dc.rights | © 2009 Elsevier Inc. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Engineered zinc finger protein | en |
dc.subject | DNA binding | en |
dc.subject | Modular assembly | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Base Sequence | en |
dc.subject.mesh | DNA/genetics | en |
dc.subject.mesh | DNA/metabolism | en |
dc.subject.mesh | DNA-Binding Proteins/genetics | en |
dc.subject.mesh | DNA-Binding Proteins/metabolism | en |
dc.subject.mesh | E-Box Elements | en |
dc.subject.mesh | Intracellular Signaling Peptides and Proteins/genetics | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Period Circadian Proteins | en |
dc.subject.mesh | Promoter Regions, Genetic | en |
dc.subject.mesh | Sequence Deletion | en |
dc.subject.mesh | Transcription, Genetic | en |
dc.subject.mesh | Zinc Fingers/genetics | en |
dc.subject.mesh | Zinc Fingers/physiology | en |
dc.title | Positive and negative cooperativity of modularly assembled zinc fingers. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00564395 | - |
dc.identifier.jtitle | Biochemical and biophysical research communications | en |
dc.identifier.volume | 387 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 440 | - |
dc.identifier.epage | 443 | - |
dc.relation.doi | 10.1016/j.bbrc.2009.07.059 | - |
dc.textversion | author | - |
dc.identifier.pmid | 19615972 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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