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Title: Generation of mouse-induced pluripotent stem cells with plasmid vectors.
Authors: Okita, Keisuke  kyouindb  KAKEN_id  orcid (unconfirmed)
Hong, Hyenjong
Takahashi, Kazutoshi  kyouindb  KAKEN_id
Yamanaka, Shinya  kyouindb  KAKEN_id
Author's alias: 沖田, 圭介
山中, 伸弥
Issue Date: Feb-2010
Publisher: Nature Publishing Group
Journal title: Nature protocols
Volume: 5
Issue: 3
Start page: 418
End page: 428
Abstract: Reprogramming of somatic cells into pluripotent stem cells has been reported by introducing a combination of several transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). The induced pluripotent stem (iPS) cells from patient's somatic cells could be a useful source for drug discovery and cell transplantation therapies. However, to date, most iPS cells were made using viral vectors, such as retroviruses and lentiviruses. Here we describe an alternative method to generate iPS cells from mouse embryonic fibroblasts (MEFs) by continual transfection of plasmid vectors. This protocol takes around 2 months to complete, from MEF isolation to iPS cell establishment. Although the reprogramming efficiency of this protocol is still low, the established iPS cells are most likely free from plasmid integration. This virus-free technique reduces the safety concern for iPS cell generation and application, and provides a source of cells for the investigation of the mechanisms underlying reprogramming and pluripotency.
Rights: © 2010 Nature Publishing Group, a division of Macmillan Publishers Limited.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1038/nprot.2009.231
PubMed ID: 20203661
Appears in Collections:Journal Articles

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