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ファイル | 記述 | サイズ | フォーマット | |
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nprot.2009.231.pdf | 256.23 kB | Adobe PDF | 見る/開く |
タイトル: | Generation of mouse-induced pluripotent stem cells with plasmid vectors. |
著者: | Okita, Keisuke https://orcid.org/0000-0001-5806-1090 (unconfirmed) Hong, Hyenjong Takahashi, Kazutoshi Yamanaka, Shinya |
著者名の別形: | 沖田, 圭介 山中, 伸弥 |
発行日: | Feb-2010 |
出版者: | Nature Publishing Group |
誌名: | Nature protocols |
巻: | 5 |
号: | 3 |
開始ページ: | 418 |
終了ページ: | 428 |
抄録: | Reprogramming of somatic cells into pluripotent stem cells has been reported by introducing a combination of several transcription factors (Oct3/4, Sox2, Klf4 and c-Myc). The induced pluripotent stem (iPS) cells from patient's somatic cells could be a useful source for drug discovery and cell transplantation therapies. However, to date, most iPS cells were made using viral vectors, such as retroviruses and lentiviruses. Here we describe an alternative method to generate iPS cells from mouse embryonic fibroblasts (MEFs) by continual transfection of plasmid vectors. This protocol takes around 2 months to complete, from MEF isolation to iPS cell establishment. Although the reprogramming efficiency of this protocol is still low, the established iPS cells are most likely free from plasmid integration. This virus-free technique reduces the safety concern for iPS cell generation and application, and provides a source of cells for the investigation of the mechanisms underlying reprogramming and pluripotency. |
著作権等: | © 2010 Nature Publishing Group, a division of Macmillan Publishers Limited. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/139480 |
DOI(出版社版): | 10.1038/nprot.2009.231 |
PubMed ID: | 20203661 |
出現コレクション: | 学術雑誌掲載論文等 |
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