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タイトル: | A novel L-isoleucine metabolism in Bacillus thuringiensis generating (2S,3R,4S)-4-hydroxyisoleucine, a potential insulinotropic and anti-obesity amino acid. |
著者: | Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) Kodera, Tomohiro Smirnov, Sergey V Hibi, Makoto Samsonova, Natalia N Koyama, Ryoukichi Yamanaka, Hiroyuki Mano, Junichi Kawashima, Takashi Yokozeki, Kenzo Shimizu, Sakayu |
著者名の別形: | 小川, 順 日比, 慎 |
キーワード: | L-Isoleucine dioxygenase 4-Hydroxyisoleucine NAD(+)-dependent dehydrogenase 2-Amino-3-methyl-4-ketopentanoic acid Bacillus thuringiensis TCA cycle |
発行日: | Mar-2011 |
出版者: | Springer-Verlag |
誌名: | Applied microbiology and biotechnology |
巻: | 89 |
号: | 6 |
開始ページ: | 1929 |
終了ページ: | 1938 |
抄録: | 4-Hydroxyisoleucine (HIL) found in fenugreek seeds has insulinotropic and anti-obesity effects and is expected to be a novel orally active drug for insulin-independent diabetes. Here, we show that the newly isolated strain Bacillus thuringiensis 2e2 and the closely related strain B. thuringiensis ATCC 35646 operate a novel metabolic pathway for L-isoleucine (L-Ile) via HIL and 2-amino-3-methyl-4-ketopentanoic acid (AMKP). The HIL synthesis was catalyzed stereoselectively by an α-ketoglutaric acid-dependent dioxygenase and to be useful for efficient production of a naturally occurring HIL isomer, (2S,3R,4S)-HIL. The (2S,3R,4S)-HIL was oxidized to (2S,3R)-AMKP by a NAD(+)-dependent dehydrogenase. The metabolic pathway functions as an effective bypass pathway that compensates for the incomplete tricarboxylic acid (TCA) cycle in Bacillus species and also explains how AMKP, a vitamin B(12) antimetabolite with antibiotic activity, is synthesized. These novel findings pave a new way for the commercial production of HIL and also for AMKP. |
著作権等: | The final publication is available at www.springerlink.com |
URI: | http://hdl.handle.net/2433/139589 |
DOI(出版社版): | 10.1007/s00253-010-2983-7 |
PubMed ID: | 21069315 |
出現コレクション: | 学術雑誌掲載論文等 |
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