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タイトル: | Histone3 lysine4 trimethylation regulated by the facilitates chromatin transcription complex is critical for DNA cleavage in class switch recombination. |
著者: | Stanlie, Andre Aida, Masatoshi Muramatsu, Masamichi Honjo, Tasuku ![]() ![]() Begum, Nasim A |
著者名の別形: | 本庶, 佑 |
発行日: | Dec-2010 |
出版者: | National Academy of Sciences |
誌名: | Proceedings of the National Academy of Sciences of the United States of America |
巻: | 107 |
号: | 51 |
開始ページ: | 22190 |
終了ページ: | 22195 |
抄録: | Ig class switch recombination (CSR) requires expression of activation-induced cytidine deaminase (AID) and transcription through target switch (S) regions. Here we show that knockdown of the histone chaperone facilitates chromatin transcription (FACT) completely inhibited S region cleavage and CSR in IgA-switch-inducible CH12F3-2A B cells. FACT knockdown did not reduce AID or S region transcripts but did decrease histone3 lysine4 trimethylation (H3K4me3) at both the Sμ and Sα regions. Because knockdown of FACT or H3K4 methyltransferase cofactors inhibited DNA cleavage in H3K4me3-depleted S regions, H3K4me3 may serve as a mark for recruiting CSR recombinase. These findings revealed an unexpected evolutionary conservation between CSR and meiotic recombination. |
著作権等: | ©2010 by the National Academy of Sciences この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/141990 |
DOI(出版社版): | 10.1073/pnas.1016923108 |
PubMed ID: | 21139053 |
出現コレクション: | 学術雑誌掲載論文等 |
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