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Title: Light-Induced Conformational Change and Transient Dissociation Reaction of the BLUF Photoreceptor Synechocystis PixD (Slr1694).
Authors: Tanaka, Keisuke
Nakasone, Yusuke  kyouindb  KAKEN_id
Okajima, Koji
Ikeuchi, Masahiko
Tokutomi, Satoru
Terazima, Masahide  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-6828-479X (unconfirmed)
Author's alias: 寺嶋, 正秀
Keywords: photoreceptor
BLUF
dynamics
diffusion
transient grating
Issue Date: 24-Jun-2011
Publisher: Elsevier Ltd.
Journal title: Journal of molecular biology
Volume: 409
Issue: 5
Start page: 773
End page: 785
Abstract: The light-induced reaction of the BLUF (blue light photoreceptor using flavin adenine dinucleotide) photoreceptor PixD from Synechocystis sp. PCC6803 (Slr1694) was investigated using the time-resolved transient grating method. A conformational change coupled with a volume contraction of 13 mL mol(-1) was observed with a time constant of 45 ms following photoexcitation. At a weak excitation light intensity, there were no further changes in volume and diffusion coefficient (D). The determined D-value (3.7×10(-11) m(2) s(-1)) suggests that PixD exists as a decamer in solution, and this oligomeric state was confirmed by size-exclusion chromatography and blue native polyacrylamide gel electrophoresis. Surprisingly, by increasing the excitation laser power, we observed a large increase in D with a time constant of 350 ms following the volume contraction reaction. The D-value of this photoproduct species (7.5×10(-11) m(2) s(-1)) is close to that of the PixD dimer. Combined with transient grating and size-exclusion chromatography measurements under light-illuminated conditions, the light-induced increase in D was attributed to a transient dissociation reaction of the PixD decamer to a dimer. For the M93A-mutated PixD, no volume or D-change was observed. Furthermore, we showed that the M93A mutant did not form the decamer but only the dimer in the dark state. These results indicate that the formation of the decamer and the conformational change around the Met residue are important factors that control the regulation of the downstream signal transduction by the PixD photoreceptor.
Rights: © 2011 Elsevier Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/142947
DOI(Published Version): 10.1016/j.jmb.2011.04.032
PubMed ID: 21530536
Appears in Collections:Journal Articles

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