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ファイル | 記述 | サイズ | フォーマット | |
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j.febslet.2011.06.012.pdf | 850.32 kB | Adobe PDF | 見る/開く |
タイトル: | Fluorescence ratiometric detection of ligand-induced receptor internalization using extracellular coiled-coil tag-probe labeling. |
著者: | Yano, Yoshiaki https://orcid.org/0000-0002-5557-3784 (unconfirmed) Matsuzaki, Katsumi https://orcid.org/0000-0002-0182-1690 (unconfirmed) |
著者名の別形: | 矢野, 義明 松崎, 勝巳 |
キーワード: | Endocytosis Tag–probe labeling Ratio imaging Membrane receptor High-throughput screening |
発行日: | 21-Jul-2011 |
出版者: | Elsevier BV |
誌名: | FEBS letters |
巻: | 585 |
号: | 14 |
開始ページ: | 2385 |
終了ページ: | 2388 |
抄録: | We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic receptor expressed on the cell surface was doubly labeled with pH-sensitive fluorescein and pH-insensitive tetramethylrhodamine. A significant increase in the tetramethylrhodamine-to-fluorescein fluorescence intensity ratio was observed after incubation with agonists in a concentration-dependent manner. This simple and accurate method of detecting the agonistic activity of receptors will be useful for high-throughput screening of drug candidates. |
著作権等: | © 2011 Federation of European Biochemical Societies Published by Elsevier B.V. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/145542 |
DOI(出版社版): | 10.1016/j.febslet.2011.06.012 |
PubMed ID: | 21708150 |
出現コレクション: | 学術雑誌掲載論文等 |
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