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DCフィールド | 値 | 言語 |
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dc.contributor.author | Nakamura, Tomonori | en |
dc.contributor.author | Nakagawa, Masato | en |
dc.contributor.author | Ichisaka, Tomoko | en |
dc.contributor.author | Shiota, Arufumi | en |
dc.contributor.author | Yamanaka, Shinya | en |
dc.contributor.alternative | 中村, 友紀 | ja |
dc.contributor.alternative | 山中, 伸弥 | ja |
dc.date.accessioned | 2012-01-12T05:47:43Z | - |
dc.date.available | 2012-01-12T05:47:43Z | - |
dc.date.issued | 2011-11 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | http://hdl.handle.net/2433/152301 | - |
dc.description.abstract | Many transcription factors and DNA binding proteins play essential roles in the development of organs in which they are highly and/or specifically expressed. Embryonic stem cell (ESC)-associated transcript 15-1 (ECAT15-1) and ECAT15-2, also known as developmental pluripotency-associated 4 (Dppa4) and Dppa2, respectively, are enriched in mouse ESCs and preimplantation embryos, and their genes encode homologous proteins with a common DNA binding domain known as the SAP motif. Previously, ECAT15-1 was shown to be important in lung development, while it is dispensable in early development. In this study, we generated ECAT15-2 single and ECAT15-1 ECAT15-2 double knockout (double KO) mice and found that almost all mutants, like ECAT15-1 mutants, died around birth with respiratory defects. Paradoxically, the expression of neither ECAT15-1 nor ECAT15-2 was detected in lung organogenesis. Several genes, such as Nkx2-5, Gata4, and Pitx2, were downregulated in the ECAT15-2-null lung. On the other hand, genomic DNA of these genes showed inactive chromatin statuses in ECAT15-2-null ESCs, but not in wild-type ESCs. The chromatin immunoprecipitation (ChIP) assay revealed that ECAT15-2 binds to the regulatory region of Nkx2-5 in ESCs. These data suggest that ECAT15-2 has important roles in lung development, where it is no longer expressed, by leaving epigenetic marks from earlier developmental stages. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Microbiology | en |
dc.rights | © 2011, American Society for Microbiology. | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Binding Sites/genetics | en |
dc.subject.mesh | Embryonic Stem Cells/metabolism | en |
dc.subject.mesh | Epigenesis, Genetic | en |
dc.subject.mesh | Female | en |
dc.subject.mesh | Gene Expression Regulation, Developmental | en |
dc.subject.mesh | Homeodomain Proteins/genetics | en |
dc.subject.mesh | Lung/abnormalities | en |
dc.subject.mesh | Lung/embryology | en |
dc.subject.mesh | Lung/metabolism | en |
dc.subject.mesh | Male | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Mice, Inbred C57BL | en |
dc.subject.mesh | Mice, Knockout | en |
dc.subject.mesh | Mice, Transgenic | en |
dc.subject.mesh | Nuclear Proteins/deficiency | en |
dc.subject.mesh | Nuclear Proteins/genetics | en |
dc.subject.mesh | Nuclear Proteins/physiology | en |
dc.subject.mesh | Pregnancy | en |
dc.subject.mesh | Regulatory Sequences, Nucleic Acid | en |
dc.subject.mesh | Transcription Factors/genetics | en |
dc.title | Essential roles of ECAT15-2/Dppa2 in functional lung development. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA10620925 | - |
dc.identifier.jtitle | Molecular and cellular biology | en |
dc.identifier.volume | 31 | - |
dc.identifier.issue | 21 | - |
dc.identifier.spage | 4366 | - |
dc.identifier.epage | 4378 | - |
dc.relation.doi | 10.1128/MCB.05701-11 | - |
dc.textversion | author | - |
dc.identifier.pmid | 21896782 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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