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タイトル: | PET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel [18]F-labeled biotin derivative. |
著者: | Kudo, Takashi Ueda, Masashi Konishi, Hiroaki Kawashima, Hidekazu Kuge, Yuji Mukai, Takahiro Miyano, Azusa Tanaka, Shotaro Kizaka-Kondoh, Shinae Hiraoka, Masahiro Saji, Hideo |
著者名の別形: | 上田, 真史 佐治, 英郎 |
キーワード: | Tumor hypoxia Hypoxia-inducible factor-1 (HIF-1) Oxygen-dependent degradation domain (ODD) Pretargeting [18]F-labeled biotin derivative |
発行日: | Oct-2011 |
出版者: | Springer |
誌名: | Molecular imaging and biology |
巻: | 13 |
号: | 5 |
開始ページ: | 1003 |
終了ページ: | 1010 |
抄録: | <Purpose> : We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors. <Procedures> : We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4-[18]F-fluorobenzoyl)norbiotinamide ([18]F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral [18]F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1α immunohistochemical signal. <Results> : [18]F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85 ± 0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral [18]F-FBB accumulation positively correlated with luciferase bioluminescence (R = 0.72, P < 0.05), and most of the area showing [18]F-FBB accumulation corresponded to HIF-1α-positive areas. <Conclusion> : Pretargeting with POS and [18]F-FBB is an effective approach for PET imaging of HIF-1-active areas in tumors. |
著作権等: | The final publication is available at www.springerlink.com This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/153020 |
DOI(出版社版): | 10.1007/s11307-010-0418-6 |
PubMed ID: | 20838908 |
出現コレクション: | 学術雑誌掲載論文等 |
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