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タイトル: PET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel [18]F-labeled biotin derivative.
著者: Kudo, Takashi
Ueda, Masashi
Konishi, Hiroaki
Kawashima, Hidekazu
Kuge, Yuji
Mukai, Takahiro
Miyano, Azusa
Tanaka, Shotaro
Kizaka-Kondoh, Shinae
Hiraoka, Masahiro  KAKEN_id
Saji, Hideo  kyouindb  KAKEN_id
著者名の別形: 上田, 真史
佐治, 英郎
キーワード: Tumor hypoxia
Hypoxia-inducible factor-1 (HIF-1)
Oxygen-dependent degradation domain (ODD)
Pretargeting
[18]F-labeled biotin derivative
発行日: Oct-2011
出版者: Springer Verlag
誌名: Molecular imaging and biology
巻: 13
号: 5
開始ページ: 1003
終了ページ: 1010
抄録: <Purpose> : We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors. <Procedures> : We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4-[18]F-fluorobenzoyl)norbiotinamide ([18]F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral [18]F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1α immunohistochemical signal. <Results> : [18]F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85 ± 0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral [18]F-FBB accumulation positively correlated with luciferase bioluminescence (R = 0.72, P < 0.05), and most of the area showing [18]F-FBB accumulation corresponded to HIF-1α-positive areas. <Conclusion> : Pretargeting with POS and [18]F-FBB is an effective approach for PET imaging of HIF-1-active areas in tumors.
著作権等: The final publication is available at www.springerlink.com
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/153020
DOI(出版社版): 10.1007/s11307-010-0418-6
PubMed ID: 20838908
出現コレクション:学術雑誌掲載論文等

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