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dc.contributor.authorKudo, Takashija
dc.contributor.authorUeda, Masashija
dc.contributor.authorKonishi, Hiroakija
dc.contributor.authorKawashima, Hidekazuja
dc.contributor.authorKuge, Yujija
dc.contributor.authorMukai, Takahiroja
dc.contributor.authorMiyano, Azusaja
dc.contributor.authorTanaka, Shotaroja
dc.contributor.authorKizaka-Kondoh, Shinaeja
dc.contributor.authorHiraoka, Masahiroja
dc.contributor.authorSaji, Hideoja
dc.contributor.alternative上田, 真史ja
dc.contributor.alternative佐治, 英郎ja
dc.date.accessioned2012-02-20T00:50:20Z-
dc.date.available2012-02-20T00:50:20Z-
dc.date.issued2011-10ja
dc.identifier.issn1536-1632ja
dc.identifier.urihttp://hdl.handle.net/2433/153020-
dc.description.abstract<Purpose> : We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors. <Procedures> : We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4-[18]F-fluorobenzoyl)norbiotinamide ([18]F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral [18]F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1α immunohistochemical signal. <Results> : [18]F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85 ± 0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral [18]F-FBB accumulation positively correlated with luciferase bioluminescence (R = 0.72, P < 0.05), and most of the area showing [18]F-FBB accumulation corresponded to HIF-1α-positive areas. <Conclusion> : Pretargeting with POS and [18]F-FBB is an effective approach for PET imaging of HIF-1-active areas in tumors.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherSpringer Verlagja
dc.rightsThe final publication is available at www.springerlink.comja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectTumor hypoxiaja
dc.subjectHypoxia-inducible factor-1 (HIF-1)ja
dc.subjectOxygen-dependent degradation domain (ODD)ja
dc.subjectPretargetingja
dc.subject[18]F-labeled biotin derivativeja
dc.subject.meshAnimalsja
dc.subject.meshBiotin/metabolismja
dc.subject.meshCell Line, Tumorja
dc.subject.meshFluorine Radioisotopes/metabolismja
dc.subject.meshHypoxia-Inducible Factor 1/metabolismja
dc.subject.meshImmunohistochemistryja
dc.subject.meshMiceja
dc.subject.meshOxygen/metabolismja
dc.subject.meshPositron-Emission Tomographyja
dc.subject.meshStreptavidinja
dc.titlePET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel [18]F-labeled biotin derivative.ja
dc.type.niitypeJournal Articleja
dc.identifier.ncidAA11820180ja
dc.identifier.jtitleMolecular imaging and biologyja
dc.identifier.volume13ja
dc.identifier.issue5ja
dc.identifier.spage1003ja
dc.identifier.epage1010ja
dc.relation.doi10.1007/s11307-010-0418-6ja
dc.textversionauthorja
dc.identifier.pmid20838908ja
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