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Title: A pre-targeting strategy for MR imaging of functional molecules using dendritic Gd-based contrast agents.
Authors: Sano, Kohei  KAKEN_id
Temma, Takashi
Azuma, Takashi
Nakai, Ryusuke  kyouindb  KAKEN_id
Narazaki, Michiko
Kuge, Yuji
Saji, Hideo  kyouindb  KAKEN_id
Author's alias: 天滿, 敬
佐治, 英郎
Keywords: Pre-targeting
Polyamidoamine dendrimer (PAMAM)
Membrane type-1 matrix metalloproteinase
Magnetic resonance imaging
Issue Date: Dec-2011
Publisher: Springer Verlag
Journal title: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging
Volume: 13
Issue: 6
Start page: 1196
End page: 1203
Abstract: [Purpose] : We aimed to establish a magnetic resonance imaging (MRI) protocol for the sensitive and specific imaging of functional molecules with a pre-targeting strategy utilizing the streptavidin–biotin interaction. Membrane type-1 matrix metalloproteinase (MT1-MMP) was selected as the target molecule. [Procedures] : The biotinylated polyamidoamine dendrimer (PAMAM)-based contrast agent (Bt-PAMAM-DTPA(Gd)) was prepared, and its proton relaxivity (r1) and affinity to streptavidin were evaluated. Tumor-bearing mice were pre-targeted with streptavidin-conjugated anti-MT1-MMP monoclonal antibody (mAb), streptavidin-conjugated negative control IgG, or saline and 3 days later were injected with Bt-PAMAM-DTPA(Gd) followed immediately by MRI for a period of 3 h. [Results] : High r1 (15.5 L mmol[−1] s[−1]) and 1.9-fold higher affinity than D-biotin were obtained. Significantly higher relative tumor signals were observed in mice pre-targeted with streptavidin-conjugated anti-MT1-MMP mAb (165% at 3 h vs. pre-administration) than with saline or streptavidin-conjugated negative control IgG (P < 0.0001). [Conclusions] : This pre-targeting approach can accomplish sensitive and specific in vivo MRI of functional molecules.
Rights: The final publication is available at www.springerlink.com
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/153055
DOI(Published Version): 10.1007/s11307-010-0463-1
PubMed ID: 21140232
Appears in Collections:Journal Articles

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