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タイトル: | A pre-targeting strategy for MR imaging of functional molecules using dendritic Gd-based contrast agents. |
著者: | Sano, Kohei Temma, Takashi Azuma, Takashi Nakai, Ryusuke https://orcid.org/0000-0002-3531-2608 (unconfirmed) Narazaki, Michiko Kuge, Yuji Saji, Hideo |
著者名の別形: | 天滿, 敬 佐治, 英郎 |
キーワード: | Pre-targeting Polyamidoamine dendrimer (PAMAM) Membrane type-1 matrix metalloproteinase Magnetic resonance imaging |
発行日: | Dec-2011 |
出版者: | Springer |
誌名: | Molecular imaging and biology |
巻: | 13 |
号: | 6 |
開始ページ: | 1196 |
終了ページ: | 1203 |
抄録: | [Purpose] : We aimed to establish a magnetic resonance imaging (MRI) protocol for the sensitive and specific imaging of functional molecules with a pre-targeting strategy utilizing the streptavidin–biotin interaction. Membrane type-1 matrix metalloproteinase (MT1-MMP) was selected as the target molecule. [Procedures] : The biotinylated polyamidoamine dendrimer (PAMAM)-based contrast agent (Bt-PAMAM-DTPA(Gd)) was prepared, and its proton relaxivity (r1) and affinity to streptavidin were evaluated. Tumor-bearing mice were pre-targeted with streptavidin-conjugated anti-MT1-MMP monoclonal antibody (mAb), streptavidin-conjugated negative control IgG, or saline and 3 days later were injected with Bt-PAMAM-DTPA(Gd) followed immediately by MRI for a period of 3 h. [Results] : High r1 (15.5 L mmol[−1] s[−1]) and 1.9-fold higher affinity than D-biotin were obtained. Significantly higher relative tumor signals were observed in mice pre-targeted with streptavidin-conjugated anti-MT1-MMP mAb (165% at 3 h vs. pre-administration) than with saline or streptavidin-conjugated negative control IgG (P < 0.0001). [Conclusions] : This pre-targeting approach can accomplish sensitive and specific in vivo MRI of functional molecules. |
著作権等: | The final publication is available at www.springerlink.com この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/153055 |
DOI(出版社版): | 10.1007/s11307-010-0463-1 |
PubMed ID: | 21140232 |
出現コレクション: | 学術雑誌掲載論文等 |
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