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Title: Langerhans cells are critical in epicutaneous sensitization with protein antigen via thymic stromal lymphopoietin receptor signaling.
Authors: Nakajima, Saeko  kyouindb  KAKEN_id
Igyártó, Botond Z
Honda, Tetsuya  kyouindb  KAKEN_id
Egawa, Gyohei
Otsuka, Atsushi  kyouindb  KAKEN_id
Hara-Chikuma, Mariko
Watanabe, Norihiko
Ziegler, Steven F
Tomura, Michio  KAKEN_id
Inaba, Kayo  kyouindb  KAKEN_id
Miyachi, Yoshiki
Kaplan, Daniel H
Kabashima, Kenji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-0773-0554 (unconfirmed)
Author's alias: 中島, 沙恵子
椛島, 健治
Keywords: Langerhans cell
TSLP
TSLP receptor
epicutaneous sensitization
protein antigen
Issue Date: Apr-2012
Publisher: Mosby, Inc.
Journal title: The Journal of allergy and clinical immunology
Volume: 129
Issue: 4
Start page: 1048
End page: 1055.e6
Abstract: Background: The clarification of cutaneous dendritic cell subset and the role of thymic stromal lymphopoietin (TSLP) signaling in epicutaneous sensitization with protein antigens, as in the development of atopic dermatitis, is a crucial issue. Objectives: Because TSLP is highly expressed in the vicinity of Langerhans cells (LCs), we sought to clarify our hypothesis that LCs play an essential role in epicutaneous sensitization with protein antigens through TSLP signaling. Methods: By using Langerin-diphtheria toxin receptor knock-in mice and human Langerin-diphtheria toxin A transgenic mice, we prepared mice deficient in LCs. We also prepared mice deficient in TSLP receptors in LCs by using TSLP receptor–deficient mice with bone marrow chimeric technique. We applied these mice to an ovalbumin (OVA)-induced epicutaneous sensitization model. Results: Upon the epicutaneous application of OVA, conditional LC depletion attenuated the development of clinical manifestations as well as serum OVA-specific IgE increase, OVA-specific T-cell proliferation, and IL-4 mRNA expression in the draining lymph nodes. Consistently, even in the steady state, permanent LC depletion resulted in decreased serum IgE levels, suggesting that LCs mediate the T[H]2 local environment. In addition, mice deficient in TSLP receptors on LCs abrogated the induction of OVA-specific IgE levels upon epicutaneous OVA sensitization. Conclusion: LCs initiate epicutaneous sensitization with protein antigens and induce T[H]2-type immune responses via TSLP signaling.
Rights: © 2012 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/155085
DOI(Published Version): 10.1016/j.jaci.2012.01.063
PubMed ID: 22385635
Appears in Collections:Journal Articles

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