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Title: Single strand DNA catenane synthesis using the formation of G-quadruplex structure.
Authors: Sannohe, Yuta
Sugiyama, Hiroshi  kyouindb  KAKEN_id
Author's alias: 杉山, 弘
Keywords: G-quadruplex
Catenane
DNA
Nanotechnology
Topology
Issue Date: 15-Mar-2012
Publisher: Elsevier Ltd.
Journal title: Bioorganic & medicinal chemistry
Volume: 20
Issue: 6
Start page: 2030
End page: 2034
Abstract: DNA is a good material for constructing nanostructures such as DNA origami. One of the challenges in this field is constructing a topologically complex structure. Here, we synthesized a DNA catenane through the formation of a G-quadruplex structure. The formation of the DNA catenane was investigated by gel electrophoresis. Interestingly, the synthesized DNA catenane was destroyed by heat treatment. Because conventional methods to construct DNA catenane include enzymatic ligation or chemical reactions, DNA is cyclized by covalent bond connection and never destroyed by heat treatment. To our knowledge, this is the first report of the synthesis of DNA catenane without using covalent bonds. Our novel way of synthesizing DNA catenane may be of use in easily recoverable DNA topological labeling.
Rights: © 2012 Elsevier Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/155087
DOI(Published Version): 10.1016/j.bmc.2012.01.040
PubMed ID: 22364954
Appears in Collections:Journal Articles

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