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j.jconrel.2011.12.033.pdf761.96 kBAdobe PDF見る/開く
タイトル: Implantable pneumatically actuated microsystem for renal pressure-mediated transfection in mice.
著者: Shimizu, Kazunori
Kawakami, Shigeru
Hayashi, Kouji
Mori, Yuki
Hashida, Mitsuru  kyouindb  KAKEN_id
Konishi, Satoshi
著者名の別形: 清水, 一憲
キーワード: Drug delivery
Gene transfer
Micromachining
Silicone elastomer
発行日: 10-Apr-2012
出版者: Elsevier B.V.
誌名: Journal of controlled release : official journal of the Controlled Release Society
巻: 159
号: 1
開始ページ: 85
終了ページ: 91
抄録: In vivo transfection is an important technique used in biological research and drug therapy development. Previously, we developed a renal pressure-mediated transfection method performed by pressing a kidney after an intravenous injection of naked nucleic acids. Although this is a useful method because of its safety and wide range of applications, an innovative approach for performing this method without repeatedly cutting open the abdomen is required. In this study, we developed an implantable microsystem fabricated by Micro-Electro-Mechanical Systems (MEMS) technologies for renal pressure-mediated transfection. The system consists of a polydimethylsiloxane pneumatic balloon actuator (PBA) used as an actuator to press the target kidney. The PBA of the implanted microsystem can be actuated without opening the abdomen by applying air pressure from outside the body to the pressure-supplying port via a needle. We successfully performed renal pressure-mediated transfection using the newly developed system when the implanted system was activated at 60kPa for 10s. This is the first report of an implantable MEMS-based microsystem that demonstrates in vivo transfection to a kidney using naked plasmid DNA.
著作権等: © 2012 Elsevier B.V.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/155489
DOI(出版社版): 10.1016/j.jconrel.2011.12.033
PubMed ID: 22245482
出現コレクション:学術雑誌掲載論文等

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