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srep00544.pdf | 2.04 MB | Adobe PDF | 見る/開く |
タイトル: | A synthetic small molecule for rapid induction of multiple pluripotency genes in mouse embryonic fibroblasts. |
著者: | Pandian, Ganesh N Nakano, Yusuke Sato, Shinsuke Morinaga, Hironobu Bando, Toshikazu Nagase, Hiroki Sugiyama, Hiroshi https://orcid.org/0000-0001-8923-5946 (unconfirmed) |
著者名の別形: | 杉山, 弘 |
キーワード: | Biochemistry Chemical genetics Bioinorganic chemistry Chemical biology |
発行日: | 30-Jul-2012 |
出版者: | Nature Publishing Group |
誌名: | Scientific reports |
巻: | 2 |
論文番号: | 544 |
抄録: | Cellular reprogramming involves profound alterations in genome-wide gene expression that is precisely controlled by a hypothetical epigenetic code. Small molecules have been shown to artificially induce epigenetic modifications in a sequence independent manner. Recently, we showed that specific DNA binding hairpin pyrrole-imidazole polyamides (PIPs) could be conjugated with chromatin modifying histone deacetylase inhibitors like SAHA to epigenetically activate certain pluripotent genes in mouse fibroblasts. In our steadfast progress to improve the efficiency of SAHA-PIPs, we identified a novel compound termed, δ that could dramatically induce the endogenous expression of Oct-3/4 and Nanog. Genome-wide gene analysis suggests that in just 24 h and at nM concentration, δ induced multiple pluripotency-associated genes including Rex1 and Cdh1 by more than ten-fold. δ treated MEFs also rapidly overcame the rate-limiting step of epithelial transition in cellular reprogramming by switching "[Formula: see text]" the complex transcriptional gene network. |
著作権等: | This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
URI: | http://hdl.handle.net/2433/159700 |
DOI(出版社版): | 10.1038/srep00544 |
PubMed ID: | 22848790 |
出現コレクション: | 学術雑誌掲載論文等 |
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