このアイテムのアクセス数: 26293

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
j.cell.2006.07.024.pdf1.27 MBAdobe PDF見る/開く
タイトル: Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
著者: Takahashi, Kazutoshi  kyouindb  KAKEN_id
Yamanaka, Shinya  kyouindb  KAKEN_id
著者名の別形: 高橋, 和利
山中, 伸弥
発行日: 25-Aug-2006
出版者: Elsevier Inc.
誌名: Cell
巻: 126
号: 4
開始ページ: 663
終了ページ: 676
抄録: Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
記述: This article is related to the Nobel Prize in Physiology or Medicine 2012
著作権等: Copyright © 2006 Elsevier Inc. All rights reserved.
This posting is granted exceptional permission by the owner of copyright.
URI: http://hdl.handle.net/2433/159777
DOI(出版社版): 10.1016/j.cell.2006.07.024
PubMed ID: 16904174
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。