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dc.contributor.authorTakahashi, Kazutoshien
dc.contributor.authorYamanaka, Shinyaen
dc.contributor.alternative高橋, 和利ja
dc.contributor.alternative山中, 伸弥ja
dc.date.accessioned2012-10-11T02:08:16Z-
dc.date.available2012-10-11T02:08:16Z-
dc.date.issued2006-08-25-
dc.identifier.issn0092-8674-
dc.identifier.urihttp://hdl.handle.net/2433/159777-
dc.descriptionThis article is related to the Nobel Prize in Physiology or Medicine 2012en
dc.description.abstractDifferentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier Inc.en
dc.rightsCopyright © 2006 Elsevier Inc. All rights reserved.en
dc.rightsThis posting is granted exceptional permission by the owner of copyright.en
dc.subject.meshAdulten
dc.subject.meshAnimalsen
dc.subject.meshCell Differentiation/physiologyen
dc.subject.meshCell Transplantationen
dc.subject.meshCells, Cultureden
dc.subject.meshDNA-Binding Proteins/geneticsen
dc.subject.meshDNA-Binding Proteins/metabolismen
dc.subject.meshEmbryo, Mammalian/cytologyen
dc.subject.meshEmbryo, Mammalian/physiologyen
dc.subject.meshFibroblasts/cytologyen
dc.subject.meshGene Expression Profilingen
dc.subject.meshHomeodomain Proteins/geneticsen
dc.subject.meshHomeodomain Proteins/metabolismen
dc.subject.meshHumansen
dc.subject.meshKruppel-Like Transcription Factors/geneticsen
dc.subject.meshKruppel-Like Transcription Factors/metabolismen
dc.subject.meshMiceen
dc.subject.meshMice, Nudeen
dc.subject.meshMice, Transgenicen
dc.subject.meshOctamer Transcription Factor-3/geneticsen
dc.subject.meshOctamer Transcription Factor-3/metabolismen
dc.subject.meshOligonucleotide Array Sequence Analysisen
dc.subject.meshPluripotent Stem Cells/cytologyen
dc.subject.meshPluripotent Stem Cells/physiologyen
dc.subject.meshProto-Oncogene Proteins c-myc/geneticsen
dc.subject.meshProto-Oncogene Proteins c-myc/metabolismen
dc.subject.meshSOXB1 Transcription Factorsen
dc.subject.meshTrans-Activators/geneticsen
dc.subject.meshTrans-Activators/metabolismen
dc.titleInduction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.en
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.ncidAA00600003-
dc.identifier.jtitleCellen
dc.identifier.volume126-
dc.identifier.issue4-
dc.identifier.spage663-
dc.identifier.epage676-
dc.relation.doi10.1016/j.cell.2006.07.024-
dc.textversionpublisher-
dc.identifier.pmid16904174-
dcterms.accessRightsopen access-
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