Downloads: 40579
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
j.cell.2006.07.024.pdf | 1.27 MB | Adobe PDF | View/Open |
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Takahashi, Kazutoshi | en |
dc.contributor.author | Yamanaka, Shinya | en |
dc.contributor.alternative | 高橋, 和利 | ja |
dc.contributor.alternative | 山中, 伸弥 | ja |
dc.date.accessioned | 2012-10-11T02:08:16Z | - |
dc.date.available | 2012-10-11T02:08:16Z | - |
dc.date.issued | 2006-08-25 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/2433/159777 | - |
dc.description | This article is related to the Nobel Prize in Physiology or Medicine 2012 | en |
dc.description.abstract | Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Inc. | en |
dc.rights | Copyright © 2006 Elsevier Inc. All rights reserved. | en |
dc.rights | This posting is granted exceptional permission by the owner of copyright. | en |
dc.subject.mesh | Adult | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Cell Differentiation/physiology | en |
dc.subject.mesh | Cell Transplantation | en |
dc.subject.mesh | Cells, Cultured | en |
dc.subject.mesh | DNA-Binding Proteins/genetics | en |
dc.subject.mesh | DNA-Binding Proteins/metabolism | en |
dc.subject.mesh | Embryo, Mammalian/cytology | en |
dc.subject.mesh | Embryo, Mammalian/physiology | en |
dc.subject.mesh | Fibroblasts/cytology | en |
dc.subject.mesh | Gene Expression Profiling | en |
dc.subject.mesh | Homeodomain Proteins/genetics | en |
dc.subject.mesh | Homeodomain Proteins/metabolism | en |
dc.subject.mesh | Humans | en |
dc.subject.mesh | Kruppel-Like Transcription Factors/genetics | en |
dc.subject.mesh | Kruppel-Like Transcription Factors/metabolism | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Mice, Nude | en |
dc.subject.mesh | Mice, Transgenic | en |
dc.subject.mesh | Octamer Transcription Factor-3/genetics | en |
dc.subject.mesh | Octamer Transcription Factor-3/metabolism | en |
dc.subject.mesh | Oligonucleotide Array Sequence Analysis | en |
dc.subject.mesh | Pluripotent Stem Cells/cytology | en |
dc.subject.mesh | Pluripotent Stem Cells/physiology | en |
dc.subject.mesh | Proto-Oncogene Proteins c-myc/genetics | en |
dc.subject.mesh | Proto-Oncogene Proteins c-myc/metabolism | en |
dc.subject.mesh | SOXB1 Transcription Factors | en |
dc.subject.mesh | Trans-Activators/genetics | en |
dc.subject.mesh | Trans-Activators/metabolism | en |
dc.title | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00600003 | - |
dc.identifier.jtitle | Cell | en |
dc.identifier.volume | 126 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 663 | - |
dc.identifier.epage | 676 | - |
dc.relation.doi | 10.1016/j.cell.2006.07.024 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 16904174 | - |
dcterms.accessRights | open access | - |
Appears in Collections: | Journal Articles |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.