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j.cell.2006.07.024.pdf1.27 MBAdobe PDF見る/開く
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dc.contributor.authorTakahashi, Kazutoshija
dc.contributor.authorYamanaka, Shinyaja
dc.contributor.alternative高橋, 和利ja
dc.contributor.alternative山中, 伸弥ja
dc.date.accessioned2012-10-11T02:08:16Z-
dc.date.available2012-10-11T02:08:16Z-
dc.date.issued2006-08-25-
dc.identifier.issn0092-8674ja
dc.identifier.urihttp://hdl.handle.net/2433/159777-
dc.descriptionThis article is related to the Nobel Prize in Physiology or Medicine 2012ja
dc.description.abstractDifferentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherElsevier Inc.ja
dc.rightsCopyright © 2006 Elsevier Inc. All rights reserved.ja
dc.rightsThis posting is granted exceptional permission by the owner of copyright.-
dc.subject.meshAdult-
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation/physiology-
dc.subject.meshCell Transplantation-
dc.subject.meshCells, Cultured-
dc.subject.meshDNA-Binding Proteins/genetics-
dc.subject.meshDNA-Binding Proteins/metabolism-
dc.subject.meshEmbryo, Mammalian/cytology-
dc.subject.meshEmbryo, Mammalian/physiology-
dc.subject.meshFibroblasts/cytology-
dc.subject.meshGene Expression Profiling-
dc.subject.meshHomeodomain Proteins/genetics-
dc.subject.meshHomeodomain Proteins/metabolism-
dc.subject.meshHumans-
dc.subject.meshKruppel-Like Transcription Factors/genetics-
dc.subject.meshKruppel-Like Transcription Factors/metabolism-
dc.subject.meshMice-
dc.subject.meshMice, Nude-
dc.subject.meshMice, Transgenic-
dc.subject.meshOctamer Transcription Factor-3/genetics-
dc.subject.meshOctamer Transcription Factor-3/metabolism-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshPluripotent Stem Cells/cytology-
dc.subject.meshPluripotent Stem Cells/physiology-
dc.subject.meshProto-Oncogene Proteins c-myc/genetics-
dc.subject.meshProto-Oncogene Proteins c-myc/metabolism-
dc.subject.meshSOXB1 Transcription Factors-
dc.subject.meshTrans-Activators/genetics-
dc.subject.meshTrans-Activators/metabolism-
dc.titleInduction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.ja
dc.type.niitypeJournal Articleja
dc.identifier.ncidAA00600003ja
dc.identifier.jtitleCellja
dc.identifier.volume126ja
dc.identifier.issue4ja
dc.identifier.spage663ja
dc.identifier.epage676ja
dc.relation.doi10.1016/j.cell.2006.07.024ja
dc.textversionpublisherja
dc.identifier.pmid16904174-
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