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タイトル: Eicosapentaenoic acid facilitates the folding of an outer membrane protein of the psychrotrophic bacterium, Shewanella livingstonensis Ac10.
著者: Dai, Xian-Zhu
Kawamoto, Jun
Sato, Satoshi B
Esaki, Nobuyoshi  KAKEN_id
Kurihara, Tatsuo  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7777-1378 (unconfirmed)
著者名の別形: 栗原, 達夫
キーワード: Eicosapentaenoic acid
Phospholipid
Membrane protein
Protein folding
Cold-adaptation mechanism
Bacteria
発行日: 24-Aug-2012
出版者: Elsevier Inc.
誌名: Biochemical and biophysical research communications
巻: 425
号: 2
開始ページ: 363
終了ページ: 367
抄録: Polyunsaturated fatty acids, such as eicosapentaenoic acid (EPA), are found in various cold-adapted microorganisms. We previously demonstrated that EPA-containing phospholipids (EPA-PLs) synthesized by the psychrotrophic bacterium Shewanella livingstonensis Ac10 support cell division, membrane biogenesis, and the production of membrane proteins at low temperatures. In this article, we demonstrate the effects of EPA-PLs on the folding and conformational transition of Omp74, a major outer membrane cold-inducible protein in this bacterium. Omp74 from an EPA-less mutant migrated differently from that of the parent strain on SDS-polyacrylamide gel, suggesting that EPA-PLs affect the conformation of Omp74 in vivo. To examine the effects of EPA-PLs on Omp74 protein folding, in vitro refolding of recombinant Omp74 was carried out with liposomes composed of 1, 2-dipalmitoleoyl-sn-glycero-3-phosphoglycerol and 1, 2-dipalmitoleoyl-sn-glycero-3-phosphoethanolamine (1:1molar ratio) with or without EPA-PLs as guest lipids. SDS-PAGE analysis of liposome-reconstituted Omp74 revealed more rapid folding in the presence of EPA-PLs. CD spectroscopy of Omp74 folding kinetics at 4°C showed that EPA-PLs accelerated β-sheet formation. These results suggest that EPA-PLs act as chemical chaperones, accelerating membrane insertion and secondary structure formation of Omp74 at low temperatures.
著作権等: © 2012 Elsevier Inc.
This is not the published version. Please cite only the published version.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/159938
DOI(出版社版): 10.1016/j.bbrc.2012.07.097
PubMed ID: 22842563
出現コレクション:学術雑誌掲載論文等

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