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Title: ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency.
Authors: Iwabuchi, Kumiko A
Yamakawa, Tatsuya
Sato, Yoshiko
Ichisaka, Tomoko
Takahashi, Kazutoshi  kyouindb  KAKEN_id
Okita, Keisuke  kyouindb  KAKEN_id  orcid (unconfirmed)
Yamanaka, Shinya  kyouindb  KAKEN_id
Author's alias: 岩渕, 久美子
山川, 達也
佐藤, 美子
一阪, 朋子
高橋, 和利
沖田, 圭介
山中, 伸弥
Issue Date: 26-May-2011
Publisher: Public Library of Science
Journal title: PloS one
Volume: 6
Issue: 5
Thesis number: e20461
Abstract: The principal factors that lead to proliferation and pluripotency in embryonic stem cells (ESCs) have been vigorously investigated. However, the global network of factors and their full signaling cascade is still unclear. In this study, we found that ECAT11 (L1td1) is one of the ESC-associated transcripts harboring a truncated fragment of ORF-1, a component of the L1 retrotransposable element. We generated an ECAT11 knock-in mouse by replacing its coding region with green fluorescent protein. In the early stage of development, the fluorescence was observed at the inner cell mass of blastocysts and epiblasts. Despite this specific expression, ECAT11-null mice grow normally and are fertile. In addition, ECAT11 was dispensable for both the proliferation and pluripotency of ESCs.We found rapid and robust activation of ECAT11 in fibroblasts after the forced expression of transcription factors that can give rise pluripotency in somatic cells. However, iPS cells could be established from ECAT11-null fibroblasts. Our data demonstrate the dispensability of ECAT11/L1td1 in pluripotency, despite its specific expression.
Rights: © 2011 Iwabuchi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0020461
PubMed ID: 21637830
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