このアイテムのアクセス数: 92

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
0022155411411090.pdf330.95 kBAdobe PDF見る/開く
タイトル: Accurate determination of S-phase fraction in proliferative cells by dual fluorescence and peroxidase immunohistochemistry with 5-bromo-2'-deoxyuridine (BrdU) and Ki67 antibodies.
著者: Tanaka, Rina
Tainaka, Motomi
Ota, Takumi
Mizuguchi, Naoki
Kato, Hiroyuki
Urabe, Shoichi
Chen, Yulin
Fustin, Jean-Michel  kyouindb  KAKEN_id
Yamaguchi, Yoshiaki  kyouindb  KAKEN_id
Doi, Masao  kyouindb  KAKEN_id
Hamada, Shinshichi
Okamura, Hitoshi
著者名の別形: 岡村, 均
発行日: Aug-2011
出版者: SAGE Publications
誌名: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
巻: 59
号: 8
開始ページ: 791
終了ページ: 798
抄録: To ensure the maintenance of tissues in mammals, cell loss must be balanced with cell production, the proliferative activity being different from tissue to tissue. In this article, the authors propose a new method for the quantification of the proliferative activity, defined as the S-phase fraction of actively cycling cells, by dual labeling with fluorescence and peroxidase immunohistochemistry using BrdU (marker of S-phase) and Ki67 antibodies (marker of G(1)-, S-, G(2)-, and M-phases) after a one-step antigen retrieval. In the generative cell zones of fundic and pyloric glandular stomachs, where the majority of cells were cycling, the authors measured a proliferative activity of 31%. In the epithelium of the forestomach and the skin, where cycling cells are intermingled with G(0) and differentiated cells, proliferative activities were 21% and 13%, respectively. In the adrenal cortex, in which cycling cells were sparsely distributed, the proliferative activity reached 32%. During the regenerative process in the skin after a lesion, the proliferative activity increased in proximity to the wound. The present one-step dual-labeling method has revealed that the proliferative activity is different between tissues and depends on the physiological or pathological state.
著作権等: © 2012 by The Histochemical Society.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/161794
DOI(出版社版): 10.1369/0022155411411090
PubMed ID: 21551319
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。