|Title:||Accurate determination of S-phase fraction in proliferative cells by dual fluorescence and peroxidase immunohistochemistry with 5-bromo-2'-deoxyuridine (BrdU) and Ki67 antibodies.|
Fustin, Jean-Michel https://orcid.org/0000-0002-6200-6075 (unconfirmed)
Yamaguchi, Yoshiaki https://orcid.org/0000-0003-4126-542X (unconfirmed)
Doi, Masao https://orcid.org/0000-0001-6264-9217 (unconfirmed)
|Author's alias:||岡村, 均|
|Journal title:||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society|
|Abstract:||To ensure the maintenance of tissues in mammals, cell loss must be balanced with cell production, the proliferative activity being different from tissue to tissue. In this article, the authors propose a new method for the quantification of the proliferative activity, defined as the S-phase fraction of actively cycling cells, by dual labeling with fluorescence and peroxidase immunohistochemistry using BrdU (marker of S-phase) and Ki67 antibodies (marker of G(1)-, S-, G(2)-, and M-phases) after a one-step antigen retrieval. In the generative cell zones of fundic and pyloric glandular stomachs, where the majority of cells were cycling, the authors measured a proliferative activity of 31%. In the epithelium of the forestomach and the skin, where cycling cells are intermingled with G(0) and differentiated cells, proliferative activities were 21% and 13%, respectively. In the adrenal cortex, in which cycling cells were sparsely distributed, the proliferative activity reached 32%. During the regenerative process in the skin after a lesion, the proliferative activity increased in proximity to the wound. The present one-step dual-labeling method has revealed that the proliferative activity is different between tissues and depends on the physiological or pathological state.|
|Rights:||© 2012 by The Histochemical Society.|
This is not the published version. Please cite only the published version.
|Appears in Collections:||Journal Articles|
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