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Title: Accurate determination of S-phase fraction in proliferative cells by dual fluorescence and peroxidase immunohistochemistry with 5-bromo-2'-deoxyuridine (BrdU) and Ki67 antibodies.
Authors: Tanaka, Rina
Tainaka, Motomi
Ota, Takumi
Mizuguchi, Naoki
Kato, Hiroyuki
Urabe, Shoichi
Chen, Yulin
Fustin, Jean-Michel  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-6200-6075 (unconfirmed)
Yamaguchi, Yoshiaki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-4126-542X (unconfirmed)
Doi, Masao  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-6264-9217 (unconfirmed)
Hamada, Shinshichi
Okamura, Hitoshi
Author's alias: 岡村, 均
Issue Date: Aug-2011
Publisher: SAGE Publications
Journal title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Volume: 59
Issue: 8
Start page: 791
End page: 798
Abstract: To ensure the maintenance of tissues in mammals, cell loss must be balanced with cell production, the proliferative activity being different from tissue to tissue. In this article, the authors propose a new method for the quantification of the proliferative activity, defined as the S-phase fraction of actively cycling cells, by dual labeling with fluorescence and peroxidase immunohistochemistry using BrdU (marker of S-phase) and Ki67 antibodies (marker of G(1)-, S-, G(2)-, and M-phases) after a one-step antigen retrieval. In the generative cell zones of fundic and pyloric glandular stomachs, where the majority of cells were cycling, the authors measured a proliferative activity of 31%. In the epithelium of the forestomach and the skin, where cycling cells are intermingled with G(0) and differentiated cells, proliferative activities were 21% and 13%, respectively. In the adrenal cortex, in which cycling cells were sparsely distributed, the proliferative activity reached 32%. During the regenerative process in the skin after a lesion, the proliferative activity increased in proximity to the wound. The present one-step dual-labeling method has revealed that the proliferative activity is different between tissues and depends on the physiological or pathological state.
Rights: © 2012 by The Histochemical Society.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/161794
DOI(Published Version): 10.1369/0022155411411090
PubMed ID: 21551319
Appears in Collections:Journal Articles

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