このアイテムのアクセス数: 104

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
JVI.01659-12.pdf25.28 MBAdobe PDF見る/開く
タイトル: Differential roles of hsp70 and hsp90 in the assembly of the replicase complex of a positive-strand RNA plant virus.
著者: Mine, Akira
Hyodo, Kiwamu
Tajima, Yuri
Kusumanegara, Kusumawaty
Taniguchi, Takako
Kaido, Masanori  kyouindb  KAKEN_id
Mise, Kazuyuki  kyouindb  KAKEN_id
Taniguchi, Hisaaki
Okuno, Tetsuro  KAKEN_id
著者名の別形: 奥野, 哲郎
発行日: Nov-2012
出版者: American Society for Microbiology
誌名: Journal of virology
巻: 86
号: 22
開始ページ: 12091
終了ページ: 12104
抄録: Assembly of viral replicase complexes of eukaryotic positive-strand RNA viruses is a regulated process: multiple viral and host components must be assembled on intracellular membranes and ordered into quaternary complexes capable of synthesizing viral RNAs. However, the molecular mechanisms underlying this process are poorly understood. In this study, we used a model virus, Red clover necrotic mosaic virus (RCNMV), whose replicase complex can be detected readily as the 480-kDa functional protein complex. We found that host heat shock proteins Hsp70 and Hsp90 are required for RCNMV RNA replication and that they interact with p27, a virus-encoded component of the 480-kDa replicase complex, on the endoplasmic reticulum membrane. Using a cell-free viral translation/replication system in combination with specific inhibitors of Hsp70 and Hsp90, we found that inhibition of p27-Hsp70 interaction inhibits the formation of the 480-kDa complex but instead induces the accumulation of large complexes that are nonfunctional in viral RNA synthesis. In contrast, inhibition of p27-Hsp90 interaction did not induce such large complexes but rendered p27 incapable of binding to a specific viral RNA element, which is a critical step for the assembly of the 480-kDa replicase complex and viral RNA replication. Together, our results suggest that Hsp70 and Hsp90 regulate different steps in the assembly of the RCNMV replicase complex.
著作権等: © 2012, American Society for Microbiology.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/162959
DOI(出版社版): 10.1128/JVI.01659-12
PubMed ID: 22933272
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。