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Title: Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells
Authors: Miyazaki, Takamichi
Futaki, Sugiko
Suemori, Hirofumi  kyouindb  KAKEN_id
Taniguchi, Yukimasa
Yamada, Masashi
Kawasaki, Miwa
Hayashi, Maria
Kumagai, Hideaki
Nakatsuji, Norio  kyouindb  KAKEN_id
Sekiguchi, Kiyotoshi
Kawase, Eihachiro  kyouindb  KAKEN_id
Author's alias: 川瀬, 栄八郎
Keywords: Biological sciences
Biotechnology
Cell biology
Issue Date: 4-Dec-2012
Publisher: Nature Publishing Group
Journal title: Nature communications
Volume: 3
Thesis number: 1236
Abstract: Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.
Description: 細胞接着タンパクを用い、 安全・高効率なヒトES / iPS細胞の培養法を開発 : 幹細胞実用化に必要な品質保証・大量生産に向けて前進. 京都大学プレスリリース. 2012-12-05.
Rights: © 2012 Nature Publishing Group
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
URI: http://hdl.handle.net/2433/164626
DOI(Published Version): 10.1038/ncomms2231
PubMed ID: 23212365
Related Link: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2012/121205_1.htm
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