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タイトル: | Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells |
著者: | Miyazaki, Takamichi Futaki, Sugiko Suemori, Hirofumi https://orcid.org/0000-0002-3565-3111 (unconfirmed) Taniguchi, Yukimasa Yamada, Masashi Kawasaki, Miwa Hayashi, Maria Kumagai, Hideaki Nakatsuji, Norio Sekiguchi, Kiyotoshi Kawase, Eihachiro https://orcid.org/0000-0002-8285-5971 (unconfirmed) |
著者名の別形: | 川瀬, 栄八郎 |
キーワード: | Biological sciences Biotechnology Cell biology |
発行日: | 4-Dec-2012 |
出版者: | Nature Publishing Group |
誌名: | Nature communications |
巻: | 3 |
論文番号: | 1236 |
抄録: | Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications. |
記述: | 細胞接着タンパクを用い、 安全・高効率なヒトES / iPS細胞の培養法を開発 : 幹細胞実用化に必要な品質保証・大量生産に向けて前進. 京都大学プレスリリース. 2012-12-05. |
著作権等: | © 2012 Nature Publishing Group This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
URI: | http://hdl.handle.net/2433/164626 |
DOI(出版社版): | 10.1038/ncomms2231 |
PubMed ID: | 23212365 |
関連リンク: | http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2012/121205_1.htm |
出現コレクション: | 学術雑誌掲載論文等 |
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