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dc.contributor.authorMiyazaki, Takamichien
dc.contributor.authorFutaki, Sugikoen
dc.contributor.authorSuemori, Hirofumien
dc.contributor.authorTaniguchi, Yukimasaen
dc.contributor.authorYamada, Masashien
dc.contributor.authorKawasaki, Miwaen
dc.contributor.authorHayashi, Mariaen
dc.contributor.authorKumagai, Hideakien
dc.contributor.authorNakatsuji, Norioen
dc.contributor.authorSekiguchi, Kiyotoshien
dc.contributor.authorKawase, Eihachiroen
dc.contributor.alternative川瀬, 栄八郎ja
dc.date.accessioned2012-12-11T04:20:18Z-
dc.date.available2012-12-11T04:20:18Z-
dc.date.issued2012-12-04-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/2433/164626-
dc.description細胞接着タンパクを用い、 安全・高効率なヒトES / iPS細胞の培養法を開発 : 幹細胞実用化に必要な品質保証・大量生産に向けて前進. 京都大学プレスリリース. 2012-12-05.ja
dc.description.abstractHuman embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherNature Publishing Groupen
dc.rights© 2012 Nature Publishing Groupen
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/en
dc.subjectBiological sciencesen
dc.subjectBiotechnologyen
dc.subjectCell biologyen
dc.titleLaminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cellsen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleNature communicationsen
dc.identifier.volume3-
dc.relation.doi10.1038/ncomms2231-
dc.textversionpublisher-
dc.identifier.artnum1236-
dc.identifier.pmid23212365-
dc.relation.urlhttp://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2012/121205_1.htm-
dcterms.accessRightsopen access-
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