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Title: Ligand binding of PDZ domains has various roles in the synaptic clustering of SAP102 and PSD-95.
Authors: Minatohara, Keiichiro
Ichikawa, Sho-Hei
Seki, Tatsuya
Fujiyoshi, Yoshinori
Doi, Tomoko  kyouindb  KAKEN_id
Author's alias: 土井, 知子
Keywords: SAP102
PDZ domain
NMDA receptor
Postsynaptic density
Synaptic clustering
Issue Date: 15-Jan-2013
Publisher: Elsevier Ireland Ltd.
Journal title: Neuroscience letters
Volume: 533
Start page: 44
End page: 49
Abstract: Synapse-associated protein 102 (SAP102) and postsynaptic density-95 (PSD-95) bind to NMDA receptors through PDZ domains and cluster at excitatory postsynaptic sites called postsynaptic densities (PSD). We previously reported that PSD-95 containing mutated PDZ domains incapable of ligand binding clustered at synaptic sites with reduced efficiency. Here, we compared the synaptic clustering of the same series of full-length SAP102 mutants in hippocampal neurons. Unexpectedly, ligand-binding deficient mutant SAP102 showed more efficient synaptic localization than wild-type SAP102. Further, when SAP102-PDZ mutants were co-expressed with either the GluN2A or GluN2B NMDA receptor subunit, both subunits showed decreased synaptic clustering, although the mutants were efficiently targeted to the synapses. This finding suggests that direct binding of NMDA receptors with SAP102 is involved in the efficient targeting of NMDA receptors to the synapses, whereas ligand binding of the PDZ domains is not essential for the synaptic clustering of SAP102.
Rights: © 2012 Elsevier Ireland Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1016/j.neulet.2012.11.019
PubMed ID: 23178474
Appears in Collections:Journal Articles

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