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dc.contributor.authorMinatohara, Keiichiroja
dc.contributor.authorIchikawa, Sho-Heija
dc.contributor.authorSeki, Tatsuyaja
dc.contributor.authorFujiyoshi, Yoshinorija
dc.contributor.authorDoi, Tomokoja
dc.contributor.alternative土井, 知子ja
dc.date.accessioned2013-01-25T04:43:18Z-
dc.date.available2013-01-25T04:43:18Z-
dc.date.issued2013-01-15ja
dc.identifier.issn0304-3940ja
dc.identifier.urihttp://hdl.handle.net/2433/169030-
dc.description.abstractSynapse-associated protein 102 (SAP102) and postsynaptic density-95 (PSD-95) bind to NMDA receptors through PDZ domains and cluster at excitatory postsynaptic sites called postsynaptic densities (PSD). We previously reported that PSD-95 containing mutated PDZ domains incapable of ligand binding clustered at synaptic sites with reduced efficiency. Here, we compared the synaptic clustering of the same series of full-length SAP102 mutants in hippocampal neurons. Unexpectedly, ligand-binding deficient mutant SAP102 showed more efficient synaptic localization than wild-type SAP102. Further, when SAP102-PDZ mutants were co-expressed with either the GluN2A or GluN2B NMDA receptor subunit, both subunits showed decreased synaptic clustering, although the mutants were efficiently targeted to the synapses. This finding suggests that direct binding of NMDA receptors with SAP102 is involved in the efficient targeting of NMDA receptors to the synapses, whereas ligand binding of the PDZ domains is not essential for the synaptic clustering of SAP102.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherElsevier Ireland Ltd.ja
dc.rights© 2012 Elsevier Ireland Ltd.ja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectSAP102ja
dc.subjectPSD-MAGUKja
dc.subjectPDZ domainja
dc.subjectNMDA receptorja
dc.subjectPostsynaptic densityja
dc.subjectSynaptic clusteringja
dc.titleLigand binding of PDZ domains has various roles in the synaptic clustering of SAP102 and PSD-95.ja
dc.type.niitypeJournal Articleja
dc.identifier.ncidAA00754925ja
dc.identifier.jtitleNeuroscience lettersja
dc.identifier.volume533ja
dc.identifier.spage44ja
dc.identifier.epage49ja
dc.relation.doi10.1016/j.neulet.2012.11.019ja
dc.textversionauthorja
dc.identifier.pmid23178474ja
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