ダウンロード数: 324
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.neulet.2012.11.019.pdf | 645.75 kB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Minatohara, Keiichiro | en |
dc.contributor.author | Ichikawa, Sho-Hei | en |
dc.contributor.author | Seki, Tatsuya | en |
dc.contributor.author | Fujiyoshi, Yoshinori | en |
dc.contributor.author | Doi, Tomoko | en |
dc.contributor.alternative | 土井, 知子 | ja |
dc.date.accessioned | 2013-01-25T04:43:18Z | - |
dc.date.available | 2013-01-25T04:43:18Z | - |
dc.date.issued | 2013-01-15 | - |
dc.identifier.issn | 0304-3940 | - |
dc.identifier.uri | http://hdl.handle.net/2433/169030 | - |
dc.description.abstract | Synapse-associated protein 102 (SAP102) and postsynaptic density-95 (PSD-95) bind to NMDA receptors through PDZ domains and cluster at excitatory postsynaptic sites called postsynaptic densities (PSD). We previously reported that PSD-95 containing mutated PDZ domains incapable of ligand binding clustered at synaptic sites with reduced efficiency. Here, we compared the synaptic clustering of the same series of full-length SAP102 mutants in hippocampal neurons. Unexpectedly, ligand-binding deficient mutant SAP102 showed more efficient synaptic localization than wild-type SAP102. Further, when SAP102-PDZ mutants were co-expressed with either the GluN2A or GluN2B NMDA receptor subunit, both subunits showed decreased synaptic clustering, although the mutants were efficiently targeted to the synapses. This finding suggests that direct binding of NMDA receptors with SAP102 is involved in the efficient targeting of NMDA receptors to the synapses, whereas ligand binding of the PDZ domains is not essential for the synaptic clustering of SAP102. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Ireland Ltd. | en |
dc.rights | © 2012 Elsevier Ireland Ltd. | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject | SAP102 | en |
dc.subject | PSD-MAGUK | en |
dc.subject | PDZ domain | en |
dc.subject | NMDA receptor | en |
dc.subject | Postsynaptic density | en |
dc.subject | Synaptic clustering | en |
dc.title | Ligand binding of PDZ domains has various roles in the synaptic clustering of SAP102 and PSD-95. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00754925 | - |
dc.identifier.jtitle | Neuroscience letters | en |
dc.identifier.volume | 533 | - |
dc.identifier.spage | 44 | - |
dc.identifier.epage | 49 | - |
dc.relation.doi | 10.1016/j.neulet.2012.11.019 | - |
dc.textversion | author | - |
dc.identifier.pmid | 23178474 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。