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タイトル: | Direct Immunochemiluminescent Assay for proBNP and Total BNP in Human Plasma proBNP and Total BNP Levels in Normal and Heart Failure. |
著者: | Nishikimi, Toshio Okamoto, Hiroyuki Nakamura, Masahiro Ogawa, Naoko Horii, Kazukiyo Nagata, Kiyoshi Nakagawa, Yasuaki Kinoshita, Hideyuki Yamada, Chinatsu Nakao, Kazuhiro Minami, Takeya Kuwabara, Yoshihiro Kuwahara, Koichiro Masuda, Izuru Kangawa, Kenji Minamino, Naoto Nakao, Kazuwa |
著者名の別形: | 錦見, 俊雄 |
発行日: | 24-Jan-2013 |
出版者: | Public Library of Science |
誌名: | PloS one |
巻: | 8 |
号: | 1 |
論文番号: | e53233 |
抄録: | [Background]Recent studies have shown that in addition to brain (or B-type) natriuretic peptide (BNP) and the N-terminal proBNP fragment, levels of intact proBNP are also increased in heart failure. Moreover, present BNP immunoassays also measure proBNP, as the anti-BNP antibody cross-reacts with proBNP. It is important to know the exact levels of proBNP in heart failure, because elevation of the low-activity proBNP may be associated with the development of heart failure. [Methodology/Principal Findings]We therefore established a two-step immunochemiluminescent assay for total BNP (BNP+proBNP) and proBNP using monoclonal antibodies and glycosylated proBNP as a standard. The assay enables measurement of plasma total BNP and proBNP within only 7 h, without prior extraction of the plasma. The detection limit was 0.4 pmol/L for a 50-µl plasma sample. Within-run CVs ranged from 5.2%–8.0% in proBNP assay and from 7.0%–8.4% in total BNP assay, and between-run CVs ranged from 5.3–7.4% in proBNP assay and from 2.9%–9.5% in total BNP assay, respectively. The dilution curves for plasma samples showed good linearity (correlation coefficients = 0.998–1.00), and analytical recovery was 90–101%. The mean total BNP and proBNP in plasma from 116 healthy subjects were 1.4±1.2 pM and 1.0±0.7 pM, respectively, and were 80±129 pM and 42±70 pM in 32 heart failure patients. Plasma proBNP levels significantly correlate with age in normal subjects. [Conclusions/Significance]Our immunochemiluminescent assay is sufficiently rapid and precise for routine determination of total BNP and proBNP in human plasma. |
著作権等: | © 2013 Nishikimi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/169792 |
DOI(出版社版): | 10.1371/journal.pone.0053233 |
PubMed ID: | 23365636 |
出現コレクション: | 学術雑誌掲載論文等 |
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