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Title: Dual modulation of the T-cell receptor-activated signal transduction pathway by morphine in human T lymphocytes.
Authors: Mizota, Toshiyuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Tsujikawa, Hiroshi
Shoda, Takehiro  kyouindb  KAKEN_id
Fukuda, Kazuhiko  kyouindb  KAKEN_id
Author's alias: 溝田, 敏幸
Keywords: Morphine
Nuclear factor-κB
Opioid-induced immunomodulation
Issue Date: Feb-2013
Publisher: Springer Japan
Journal title: Journal of anesthesia
Volume: 27
Issue: 1
Start page: 80
End page: 87
Abstract: [Purpose]In this study, we aimed to investigate the effect of morphine on the activation of extracellular signal-regulated kinase (ERK) and nuclear factor-κB (NF-κB), both of which play crucial roles in T-cell activation. [Methods]Human CD3+ T cells and Jurkat T cells were stimulated by anti-CD3 antibody or phorbol 12-myristate 13-acetate plus ionomycin with or without 24-h pretreatment with morphine. Activation of ERK was assessed by immunoblot analysis of phosphorylated ERK. Activation of the NF-κB signaling pathway was examined by analyzing nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) phosphorylation using immunoblotting, and interleukin-2 (IL-2) gene expression using quantitative real-time reverse-transcriptase polymerase chain reaction. [Results]Morphine pretreatment enhanced ERK phosphorylation, but inhibited IκBα phosphorylation and IL-2 gene expression in activated T cells. The effects of morphine on ERK phosphorylation and IL-2 gene expression were not antagonized by naloxone. We detected κ-opioid receptor transcript in T cells, but U50, 488, a κ-receptor-selective agonist, did not enhance ERK phosphorylation. [Conclusion]Morphine enhances ERK signaling, whereas it inhibits NF-κB signaling in activated human T cells. These effects of morphine are unlikely to be mediated by known opioid receptors.
Rights: The final publication is available at
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1007/s00540-012-1472-9
PubMed ID: 22932814
Appears in Collections:Journal Articles

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