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タイトル: | Amperometric biosensor based on reductive H2O2 detection using pentacyanoferrate-bound polymer for creatinine determination. |
著者: | Nieh, Chi-Hua Tsujimura, Seiya Shirai, Osamu Kano, Kenji |
著者名の別形: | 加納, 健司 |
キーワード: | Pentacyanoferrate-bound poly(1-vinylimidazole) Creatinine amidohydrolase Reductive H2O2 detection Peroxidase Nafion Urine creatinine test |
発行日: | 12-Mar-2013 |
出版者: | Elsevier B.V. |
誌名: | Analytica chimica acta |
巻: | 767 |
開始ページ: | 128 |
終了ページ: | 133 |
抄録: | Pentacyanoferrate-bound poly(1-vinylimidazole) (PVI[Fe(CN)5]) was selected as a mediator for amperometric creatinine determination based on the reductive H2O2 detection. Creatinine amidohydrolase (CNH), creatine amidohydrolase (CRH), sarcosine oxidase (SOD), peroxidase (POD), and PVI[Fe(CN)5] were crosslinked with poly(ethylene glycol) diglycidyl ether (PEGDGE) on a glassy carbon (GC) electrode for a creatinine biosensor fabrication. Reduction current was monitored at −0.1 V in the presence of creatinine and O2. It is revealed that PVI[Fe(CN)5] is suitable as a mediator for a bioelectrocatalytic reaction of POD, since PVI[Fe(CN)5] neither reacts with reactants nor works as an electron acceptor of SOD. The amounts of PVI[Fe(CN)5], PEGDGE, and enzymes were optimized toward creatinine detection. Nafion as a protecting film successfully prevented the enzyme layer from interferences. The detection limit and linear range in creatinine determination were 12 μM and 12–500 μM (R[2]= 0.993), respectively, and the sensitivity was 11 mA cm[−2] M[−1], which is applicable for urine creatinine tests. The results of the creatinine determination for four urine samples measured with this proposed method were compared with Jaffe method, and a good correlation was obtained between the results. |
著作権等: | © 2013 Elsevier B.V. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/173085 |
DOI(出版社版): | 10.1016/j.aca.2012.12.052 |
PubMed ID: | 23452796 |
出現コレクション: | 学術雑誌掲載論文等 |
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