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Title: | Wortmannin efficiently suppresses the recovery from radiation-induced damage in pimonidazole-unlabeled quiescent tumor cell population. |
Authors: | Masunaga, Shin-Ichiro ![]() Sakurai, Yoshinori ![]() ![]() ![]() Tanaka, Hiroki ![]() ![]() Suzuki, Minoru ![]() ![]() ![]() Kondo, Natsuko ![]() ![]() Narabayashi, Masaru ![]() Maruhashi, Akira Ono, Koji |
Author's alias: | 増永, 慎一郎 |
Keywords: | Quiescent cell recovery from radiation-induced damage pimonidazole wortmannin caffeine |
Issue Date: | Mar-2013 |
Publisher: | Oxford University Press |
Journal title: | Journal of radiation research |
Volume: | 54 |
Issue: | 2 |
Start page: | 221 |
End page: | 229 |
Abstract: | Labeling of proliferating (P) cells in mice bearing EL4 tumors was achieved by continuous administration of 5-bromo-2'-deoxyuridine (BrdU). Tumors were irradiated with γ-rays at 1 h after pimonidazole administration followed by caffeine or wortmannin treatment. Twenty-four hours later, assessment of the responses of quiescent (Q) and total (= P + Q) cell populations were based on the frequencies of micronucleation and apoptosis using immunofluorescence staining for BrdU. The response of the pimonidazole-unlabeled tumor cell fractions was assessed by means of apoptosis frequency using immunofluorescence staining for pimonidazole. The pimonidazole-unlabeled cell fraction showed significantly enhanced radio-sensitivity compared with the whole cell fraction more remarkably in Q cells than total cells. However, a significantly greater decrease in radio-sensitivity in the pimonidazole-unlabeled than the whole cell fraction, evaluated using an assay performed 24 hours after irradiation, was more clearly observed in Q cells than total cells. In both the pimonidazole-unlabeled and the whole cell fractions, wortmannin efficiently suppressed the reduction in sensitivity due to delayed assay. Wortmannin combined with γ-ray irradiation is useful for suppressing the recovery from radiation-induced damage especially in the pimonidazole-unlabeled cell fraction within the total and Q tumor cell populations. |
Rights: | © The Author 2012. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited |
URI: | http://hdl.handle.net/2433/173088 |
DOI(Published Version): | 10.1093/jrr/rrs094 |
PubMed ID: | 23097299 |
Appears in Collections: | Journal Articles |

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