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タイトル: | Simple and longstanding adipose tissue engineering in rabbits. |
著者: | Tsuji, Wakako Inamoto, Takashi Ito, Ran Morimoto, Naoki https://orcid.org/0000-0002-0712-3172 (unconfirmed) Tabata, Yasuhiko Toi, Masakazu https://orcid.org/0000-0003-1488-9958 (unconfirmed) |
著者名の別形: | 辻, 和香子 |
キーワード: | Adipose tissue engineering Type I collagen sponge Long period Rabbits Ultrasonography |
発行日: | Mar-2013 |
出版者: | Springer Japan |
誌名: | Journal of artificial organs |
巻: | 16 |
号: | 1 |
開始ページ: | 110 |
終了ページ: | 114 |
抄録: | Adipose tissue engineering for breast reconstruction can be performed for patients who have undergone breast surgery. We have previously confirmed adipogenesis in mice implanted with type I collagen sponge with controlled release of fibroblast growth factor 2 (FGF2) and human adipose tissue-derived stem cells. However, in order to use this approach to treat breast cancer patients, a large amount of adipose tissue is needed, and FGF2 is not readily available. Thus, we aimed to regenerate large amounts of adipose tissue without FGF2 for a long period. Under general anesthesia, cages made of polypropylene mesh were implanted into the rabbits' bilateral fat pads. Each cage was 10 mm in radius and 10 mm in height. Minced type I collagen sponge was injected as a scaffold into the cage. Regenerated tissue in the cage was examined with ultrasonography, and the cages were harvested 3, 6, and 12 months after the implantation. Ultrasonography revealed a gradually increasing homogeneous high-echo area in the cage. Histology of the specimen was assessed with hematoxylin and eosin staining. The percentages of regenerated adipose tissue area were 76.2 ± 13.0 and 92.8 ± 6.6 % at 6 and 12 months after the implantation, respectively. Our results showed de novo adipogenesis 12 months after the implantation of only type I collagen sponge inside the space. Ultrasonography is a noninvasive and useful method of assessing the growth of the tissue inside the cage. This simple method could be a promising clinical modality in breast reconstruction. |
著作権等: | The final publication is available at www.springerlink.com この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/173128 |
DOI(出版社版): | 10.1007/s10047-012-0670-4 |
PubMed ID: | 23114565 |
出現コレクション: | 学術雑誌掲載論文等 |
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