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Title: Simple and longstanding adipose tissue engineering in rabbits.
Authors: Tsuji, Wakako
Inamoto, Takashi
Ito, Ran  kyouindb  KAKEN_id
Morimoto, Naoki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-0712-3172 (unconfirmed)
Tabata, Yasuhiko  kyouindb  KAKEN_id
Toi, Masakazu  kyouindb  KAKEN_id
Author's alias: 辻, 和香子
Keywords: Adipose tissue engineering
Type I collagen sponge
Long period
Rabbits
Ultrasonography
Issue Date: Mar-2013
Publisher: Springer Japan
Journal title: Journal of artificial organs
Volume: 16
Issue: 1
Start page: 110
End page: 114
Abstract: Adipose tissue engineering for breast reconstruction can be performed for patients who have undergone breast surgery. We have previously confirmed adipogenesis in mice implanted with type I collagen sponge with controlled release of fibroblast growth factor 2 (FGF2) and human adipose tissue-derived stem cells. However, in order to use this approach to treat breast cancer patients, a large amount of adipose tissue is needed, and FGF2 is not readily available. Thus, we aimed to regenerate large amounts of adipose tissue without FGF2 for a long period. Under general anesthesia, cages made of polypropylene mesh were implanted into the rabbits' bilateral fat pads. Each cage was 10 mm in radius and 10 mm in height. Minced type I collagen sponge was injected as a scaffold into the cage. Regenerated tissue in the cage was examined with ultrasonography, and the cages were harvested 3, 6, and 12 months after the implantation. Ultrasonography revealed a gradually increasing homogeneous high-echo area in the cage. Histology of the specimen was assessed with hematoxylin and eosin staining. The percentages of regenerated adipose tissue area were 76.2 ± 13.0 and 92.8 ± 6.6 % at 6 and 12 months after the implantation, respectively. Our results showed de novo adipogenesis 12 months after the implantation of only type I collagen sponge inside the space. Ultrasonography is a noninvasive and useful method of assessing the growth of the tissue inside the cage. This simple method could be a promising clinical modality in breast reconstruction.
Rights: The final publication is available at www.springerlink.com
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/173128
DOI(Published Version): 10.1007/s10047-012-0670-4
PubMed ID: 23114565
Appears in Collections:Journal Articles

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