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Title: Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium Microcystis aeruginosa in western Japan
Authors: Ohbayashi, Kako
Hodoki, Yoshikuni
Kobayashi, Yuki
Okuda, Noboru  KAKEN_id
Nakano, Shin-Ichi  kyouindb  KAKEN_id
Author's alias: 中野, 伸一
Keywords: ITS region
genotypic diversity
harmful algae
Microcystis
Issue Date: Apr-2013
Publisher: NRC Research Press, Canadian Science Publishing
Journal title: Canadian journal of microbiology
Volume: 59
Issue: 4
Start page: 266
End page: 272
Abstract: Microcystis aeruginosa is one of the bloom-forming harmful algae in freshwater ecosystems. We genetically characterized Microcystis populations during bloom-forming periods in various reservoirs, lakes, and ponds in Japan during 2009. Using phylogenetic analysis, we evaluated the relationship between current genotype expansions and geographic location within western Japan and intraspecific variation. Microcystis aeruginosa colonies were isolated at 15 sites and were analyzed by sequencing the 16S-23S internal transcribed spacer (ITS) region of the ribosomal operon, and the potential to produce toxins was assessed by PCR-based detection of the microcystin synthetase gene mcyG. In total, 171 colonies were separated into 41 genotypes. The highest genotypic composition was detected in the south basin of Lake Biwa and the lowest in Lagoon Iba. Cluster analysis indicated no obvious association between genotypic composition and geographic distance. Thus, clear genetic differentiation accompanied by geographic origins was not found in western Japan. The resulting neighbor-joining tree revealed 3 clusters, 2 of which contained strains that showed both nonamplification and amplification of the mcyG gene.
Rights: © Copyright 2013 – Canadian Science Publishing.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/173931
DOI(Published Version): 10.1139/cjm-2012-0724
PubMed ID: 23586751
Appears in Collections:Journal Articles

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