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1061186X.2011.633259.pdf | 368.19 kB | Adobe PDF | 見る/開く |
タイトル: | Ultrasound-responsive thrombus treatment with zinc-stabilized gelatin nano-complexes of tissue-type plasminogen activator. |
著者: | Uesugi, Yoshiko Kawata, Hiroyuki Saito, Yoshihiko Tabata, Yasuhiko |
著者名の別形: | 田畑, 泰彦 |
キーワード: | t-PA thrombolytic therapy gelatin metal ions ultrasound |
発行日: | Apr-2012 |
出版者: | Informa Healthcare |
誌名: | Journal of drug targeting |
巻: | 20 |
号: | 3 |
開始ページ: | 224 |
終了ページ: | 234 |
抄録: | This study is undertaken to design zinc-stabilized gelatin nano-complexes of tissue-type plasminogen activator (t-PA) for thrombolytic therapy where the t-PA activity can be recovered in the blood circulation upon ultrasound irradiation. Various molecular weights of gelatin were complexed with t-PA by their simply mixing in aqueous solution. Then, zinc acetate, calcium acetate or magnesium acetate was added to form nano-sized gelatin-t-PA complexes. The complexes had the apparent molecular size of about 100 nm. When zinc ions were added to the gelatin-t-PA complexes, the t-PA activity was suppressed most strongly to 57% of the original, free t-PA activity. Upon ultrasound exposure in vitro, the t-PA activity was fully recovered. A cell culture experiment with L929 fibroblasts demonstrated no cytotoxicity of complexes at the concentration used for the in vivo experiment. The half-life of t-PA in the blood circulation prolonged by the complexation with gelatin and zinc ions. The zinc-stabilized t-PA-gelatin complex is a promising t-PA delivery system which can manipulate the thrombolytic activity by the local ultrasound irradiation. |
著作権等: | © 2012 Informa Plc. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/174341 |
DOI(出版社版): | 10.3109/1061186X.2011.633259 |
PubMed ID: | 22087861 |
出現コレクション: | 学術雑誌掲載論文等 |
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