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Title: Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p–Pex21p
Authors: Pan, Dongqing  kyouindb  KAKEN_id  orcid (unconfirmed)
Nakatsu, Toru  kyouindb  KAKEN_id  orcid (unconfirmed)
Kato, Hiroaki  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 加藤, 博章
Issue Date: 30-Jun-2013
Publisher: Nature Publishing Group
Journal title: Nature Structural & Molecular Biology
Abstract: Appropriate targeting of matrix proteins to peroxisomes is mainly directed by two types of peroxisomal targeting signals, PTS1 and PTS2. Although the basis of PTS1 recognition has been revealed by structural studies, that of PTS2 recognition remains elusive. Here we present the crystal structure of a heterotrimeric PTS2-recognition complex from Saccharomyces cerevisiae, containing Pex7p, the C-terminal region of Pex21p and the PTS2 of the peroxisomal 3-ketoacyl-CoA thiolase. Pex7p forms a β-propeller structure and provides a platform for cooperative interactions with both the amphipathic PTS2 helix and Pex21p. The C-terminal region of Pex21p directly covers the hydrophobic surfaces of both Pex7p and PTS2, and the resulting hydrophobic core is the primary determinant of stable complex formation. Together with in vivo and in vitro functional assays of Pex7p and Pex21p variants, our findings reveal the molecular mechanism of PTS2 recognition.
Description: ペルオキシソーム病RCDP-1の分子機構の解明.京都大学プレスリリース.2013-07-01.
Rights: © 2013 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1038/nsmb.2618
PubMed ID: 23812376
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