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Title: Cholestasis induces reversible accumulation of periplakin in mouse liver.
Authors: Ito, Shinji  kyouindb  KAKEN_id
Satoh, Junko
Matsubara, Tsutomu
Shah, Yatrik M
Ahn, Sung-Hoon
Anderson, Cherie R
Shan, Weiwei
Peters, Jeffrey M
Gonzalez, Frank J
Author's alias: 伊藤, 慎二
Keywords: Periplakin
Bile acids
Farnesoid X receptor
Urinary stasis
Issue Date: Jul-2013
Publisher: BioMed Central Ltd.
Journal title: BMC gastroenterology
Volume: 13
Thesis number: 116
Abstract: [Background]Periplakin (PPL) is a rod-shaped cytolinker protein thought to connect cellular adhesion junctional complexes to cytoskeletal filaments. PPL serves as a structural component of the cornified envelope in the skin and interacts with various types of proteins in cultured cells; its level decreases dramatically during tumorigenic progression in human epithelial tissues. Despite these intriguing observations, the physiological roles of PPL, especially in non-cutaneous tissues, are still largely unknown. Because we observed a marked fluctuation of PPL expression in mouse liver in association with the bile acid receptor farnesoid X receptor (FXR) and cholestasis, we sought to characterize the role of PPL in the liver and determine its contributions to the etiology and pathogenesis of cholestasis. [Methods]Time- and context-dependent expression of PPL in various mouse models of hepatic and renal disorders were examined by immunohistochemistry, western blotting, and quantitative real-time polymerase chain reactions. [Results]The hepatic expression of PPL was significantly decreased in Fxr−/− mice. In contrast, the expression was dramatically increased during cholestasis, with massive PPL accumulation observed at the boundaries of hepatocytes in wild-type mice. Interestingly, the hepatic accumulation of PPL resulting from cholestasis was reversible. In addition, similar accumulation of PPL at cellular boundaries was found in epithelial cells around renal tubules upon ureteral obstruction. [Conclusions]PPL may be involved in the temporal accommodation to fluid stasis in different tissues. Further examination of the roles for PPL may lead to the discovery of a novel mechanism for cellular protection by cytolinkers that is applicable to many tissues and in many contexts.
Rights: © 2013 Ito et al.; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI(Published Version): 10.1186/1471-230X-13-116
PubMed ID: 23849208
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