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Title: Emergence and spread of B2-ST131-O25b, B2-ST131-O16 and D-ST405 clonal groups among extended-spectrum-β-lactamase-producing Escherichia coli in Japan.
Authors: Matsumura, Yasufumi  kyouindb  KAKEN_id  orcid (unconfirmed)
Yamamoto, Masaki  kyouindb  KAKEN_id
Nagao, Miki  kyouindb  KAKEN_id  orcid (unconfirmed)
Hotta, Gou
Matsushima, Aki  kyouindb  KAKEN_id
Ito, Yutaka
Takakura, Shunji  kyouindb  KAKEN_id
Ichiyama, Satoshi  kyouindb  KAKEN_id
Author's alias: 松村, 康史
Keywords: ESBLs
clonal genotypes
sequence types
Issue Date: 26-Jul-2012
Publisher: Oxford University Press
Journal title: The Journal of antimicrobial chemotherapy
Volume: 67
Issue: 11
Start page: 2612
End page: 2620
Abstract: [Objectives ]The increasing prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has been associated with the emergence of the CTX-M-producing sequence type 131 (ST131) pandemic clonal group, a member of the O25b serogroup and the B2 phylogenetic group. To assess the clonal spread of ESBL-producing E. coli in Japan, a regional surveillance programme was conducted. [Methods] A total of 581 ESBL-producing clinical specimen E. coli isolates were collected between 2001 and 2010. Clonal groups, including ST131, D-ST405, D-ST393 and D-ST69, were determined using the PCR O type, phylogenetic grouping by triplex PCR, allele-specific PCR and multilocus sequence typing (MLST). A subset of clonal groups underwent PFGE. [Results]Among clonal strains, 215 isolates (37%) were identified as belonging to the ST131 group, 185 as B2-ST131-O25b (32%), 26 as B2-ST131-O16 (4%), 3 as B1-ST131-O25b (0.5%) and 1 as B2-ST131-O-non-typeable (0.1%). Forty-one isolates (7%) were identified as belonging to the D-ST405 clonal group, seven (1%) as D-ST69 and two (0.3%) as D-ST393. The B2-ST131-O16 clonal group was characterized by CTX-M-14 and a significantly lower ciprofloxacin resistance rate than the B2-ST131-O25b clonal group. The B2-ST131-O16 and B2-ST131-O25b clonal groups each made up a single PFGE cluster, with 65% similarity. The rate of ESBL-producing E. coli increased over the years (0.2% in 2001 to 9.7% in 2010) and corresponded to increases in the numbers of the B2-ST131-O25b, B2-ST131-O16 and D-ST405 clonal groups. [Conclusions] The B2-ST131-O25b, B2-ST131-O16 and D-ST405 clonal groups have contributed to the spread of ESBL-producing E. coli in Japan.
Rights: © The Author 2012. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1093/jac/dks278
PubMed ID: 22843833
Appears in Collections:Journal Articles

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