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Title: Emergence and spread of B2-ST131-O25b, B2-ST131-O16 and D-ST405 clonal groups among extended-spectrum-β-lactamase-producing Escherichia coli in Japan.
Authors: Matsumura, Yasufumi  kyouindb  KAKEN_id
Yamamoto, Masaki  kyouindb  KAKEN_id
Nagao, Miki  kyouindb  KAKEN_id
Hotta, Gou
Matsushima, Aki
Ito, Yutaka
Takakura, Shunji  kyouindb  KAKEN_id
Ichiyama, Satoshi  kyouindb  KAKEN_id
Author's alias: 松村, 康史
Keywords: ESBLs
clonal genotypes
sequence types
CTX-M-14
Issue Date: 26-Jul-2012
Publisher: Oxford University Press
Journal title: The Journal of antimicrobial chemotherapy
Volume: 67
Issue: 11
Start page: 2612
End page: 2620
Abstract: [Objectives ]The increasing prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has been associated with the emergence of the CTX-M-producing sequence type 131 (ST131) pandemic clonal group, a member of the O25b serogroup and the B2 phylogenetic group. To assess the clonal spread of ESBL-producing E. coli in Japan, a regional surveillance programme was conducted. [Methods] A total of 581 ESBL-producing clinical specimen E. coli isolates were collected between 2001 and 2010. Clonal groups, including ST131, D-ST405, D-ST393 and D-ST69, were determined using the PCR O type, phylogenetic grouping by triplex PCR, allele-specific PCR and multilocus sequence typing (MLST). A subset of clonal groups underwent PFGE. [Results]Among clonal strains, 215 isolates (37%) were identified as belonging to the ST131 group, 185 as B2-ST131-O25b (32%), 26 as B2-ST131-O16 (4%), 3 as B1-ST131-O25b (0.5%) and 1 as B2-ST131-O-non-typeable (0.1%). Forty-one isolates (7%) were identified as belonging to the D-ST405 clonal group, seven (1%) as D-ST69 and two (0.3%) as D-ST393. The B2-ST131-O16 clonal group was characterized by CTX-M-14 and a significantly lower ciprofloxacin resistance rate than the B2-ST131-O25b clonal group. The B2-ST131-O16 and B2-ST131-O25b clonal groups each made up a single PFGE cluster, with 65% similarity. The rate of ESBL-producing E. coli increased over the years (0.2% in 2001 to 9.7% in 2010) and corresponded to increases in the numbers of the B2-ST131-O25b, B2-ST131-O16 and D-ST405 clonal groups. [Conclusions] The B2-ST131-O25b, B2-ST131-O16 and D-ST405 clonal groups have contributed to the spread of ESBL-producing E. coli in Japan.
Rights: © The Author 2012. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/178038
DOI(Published Version): 10.1093/jac/dks278
PubMed ID: 22843833
Appears in Collections:Journal Articles

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