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Title: Reconstruction of Active Regular Motion in Amoeba Extract: Dynamic Cooperation between Sol and Gel States.
Authors: Nishigami, Yukinori
Ichikawa, Masatoshi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-6345-2106 (unconfirmed)
Kazama, Toshiya
Kobayashi, Ryo
Shimmen, Teruo
Yoshikawa, Kenichi
Sonobe, Seiji
Author's alias: 市川, 正敏
Issue Date: 5-Aug-2013
Publisher: Public Library of Science
Journal title: PloS one
Volume: 8
Issue: 8
Thesis number: e70317
Abstract: Amoeboid locomotion is one of the typical modes of biological cell migration. Cytoplasmic sol-gel conversion of an actomyosin system is thought to play an important role in locomotion. However, the mechanisms underlying sol-gel conversion, including trigger, signal, and regulating factors, remain unclear. We developed a novel model system in which an actomyosin fraction moves like an amoeba in a cytoplasmic extract. Rheological study of this model system revealed that the actomyosin fraction exhibits shear banding: the sol-gel state of actomyosin can be regulated by shear rate or mechanical force. Furthermore, study of the living cell indicated that the shear-banding property also causes sol-gel conversion with the same order of magnitude as that of shear rate. Our results suggest that the inherent sol-gel transition property plays an essential role in the self-regulation of autonomous translational motion in amoeba.
Rights: © 2013 Nishigami et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/178066
DOI(Published Version): 10.1371/journal.pone.0070317
PubMed ID: 23940560
Appears in Collections:Journal Articles

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