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Title: Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus.
Authors: Ebina, Hirotaka  KAKEN_id
Misawa, Naoko
Kanemura, Yuka
Koyanagi, Yoshio  kyouindb  KAKEN_id
Author's alias: 蝦名, 博貴
Keywords: DNA sequencing
Mutation
Retrovirus
Chromosomes
Issue Date: 26-Aug-2013
Publisher: Nature Publishing Group
Journal title: Scientific reports
Volume: 3
Thesis number: 2510
Abstract: Even though highly active anti-retroviral therapy is able to keep HIV-1 replication under control, the virus can lie in a dormant state within the host genome, known as a latent reservoir, and poses a threat to re-emerge at any time. However, novel technologies aimed at disrupting HIV-1 provirus may be capable of eradicating viral genomes from infected individuals. In this study, we showed the potential of the CRISPR/Cas9 system to edit the HIV-1 genome and block its expression. When LTR-targeting CRISPR/Cas9 components were transfected into HIV-1 LTR expression-dormant and -inducible T cells, a significant loss of LTR-driven expression was observed after stimulation. Sequence analysis confirmed that this CRISPR/Cas9 system efficiently cleaved and mutated LTR target sites. More importantly, this system was also able to remove internal viral genes from the host cell chromosome. Our results suggest that the CRISPR/Cas9 system may be a useful tool for curing HIV-1 infection.
Rights: © 2013 Nature Publishing Group
This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
URI: http://hdl.handle.net/2433/178737
DOI(Published Version): 10.1038/srep02510
PubMed ID: 23974631
Appears in Collections:Journal Articles

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