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Title: Direct Induction of Chondrogenic Cells from Human Dermal Fibroblast Culture by Defined Factors
Authors: Outani, Hidetatsu
Okada, Minoru
Yamashita, Akihiro  kyouindb  KAKEN_id
Nakagawa, Kanako
Yoshikawa, Hideki
Tsumaki, Noriyuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 王谷, 英達
岡田, 稔
山下, 晃弘
妻木, 範行
Issue Date: 16-Oct-2013
Publisher: Public Library of Science
Journal title: PLoS ONE
Volume: 8
Issue: 10
Thesis number: e77365
Abstract: The repair of large cartilage defects with hyaline cartilage continues to be a challenging clinical issue. We recently reported that the forced expression of two reprogramming factors (c-Myc and Klf4) and one chondrogenic factor (SOX9) can induce chondrogenic cells from mouse dermal fibroblast culture without going through a pluripotent state. We here generated induced chondrogenic (iChon) cells from human dermal fibroblast (HDF) culture with the same factors. We developed a chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells. The human iChon cells expressed marker genes for chondrocytes but not fibroblasts, and were derived from non-chondrogenic COL11A2-negative cells. The human iChon cells formed cartilage but not tumors in nude mice. This approach could lead to the preparation of cartilage directly from skin in human, without going through pluripotent stem cells.
Description: ヒトの皮膚細胞から軟骨様細胞へ直接変換に成功. 京都大学プレスリリース. 2013-10-14.
Rights: © 2013 Outani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0077365
PubMed ID: 24146984
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