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Title: The structural changes in crystalline cellulose and effects on enzymatic digestibility
Authors: Horikawa, Yoshiki
Konakahara, Naoya
Imai, Tomoya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7026-0287 (unconfirmed)
Kentaro, Abe  kyouindb  KAKEN_id
Kobayashi, Yoshinori
Sugiyama, Junji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-5388-4925 (unconfirmed)
Author's alias: 堀川, 祥生
Keywords: Crystalline polymorph
Enzymatic hydrolysis
Susceptibility
FTIR spectroscopy
Issue Date: Nov-2013
Publisher: Elsevier Ltd.
Journal title: Polymer Degradation and Stability
Volume: 98
Issue: 11
Start page: 2351
End page: 2356
Abstract: The enzymatic hydrolysis of cellulose I achieves almost complete digestion when sufficient enzyme loading as much as 20 mg/g-substrate is applied. However, the yield of digestion reaches the limit when the enzyme dosage is decreased to 2 mg/g-substrate. Therefore, we have performed three pretreatments such as mercerization, dissolution into phosphoric acid and EDA treatment. Transformation into cellulose II hydrate by mercerization and dissolution into phosphoric acid were not sufficient because substrate changed to highly crystalline structure during saccharification. On the other hand, in the case of crystalline conversion of cellulose I to IIII by EDA, almost perfect digestion was achieved even in enzyme loading as small as 0.5 mg/g-substrate, furthermore, hydrolyzed residue was typical cellulose I. The structural analysis of substrate after saccharification provides an insight into relationships between cellulose crystalline property and cellulase toward better enzymatic digestion.
Rights: © 2013 Elsevier Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/179455
DOI(Published Version): 10.1016/j.polymdegradstab.2013.08.004
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