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Title: Site-specific DNA oxidation by a dinuclear copper complex containing a photoisomerizable azobenzene ligand
Authors: Ito, Takeo
Akamatsu, Kanako
Takeuchi, Kouhei
Satani, Manami
Tanabe, Kazuhito  KAKEN_id
Nishimoto, Sei-ichi
Author's alias: 伊藤, 健雄
Keywords: Multinuclear copper complex
Photoisomerization
H_[2]O_[2]
DNA oxidation
Azobenzene
Issue Date: Nov-2013
Publisher: Elsevier B.V.
Journal title: Inorganica Chimica Acta
Volume: 408
Start page: 230
End page: 234
Abstract: A dinuclear copper complex possessing an azobenzene backbone (Cu^[II]_[2]1) was synthesized and its affinity towards DNA was investigated and found to be dependent on the trans–cis isomeric forms. Upon exposure to UV light at 365 nm the trans form of the complex (Cu^[II]_[2]trans-1) underwent photoisomerization into the cis-isomer (Cu^[II]_[2]cis-1), which reverted to the original trans-form on exposure to visible light at 420 nm. Both thetrans and cis isomers exhibited moderate DNA cleavage activity toward plasmid duplex DNA in the presence of a reducing agent 3-mercaptopropionic acid (MPA) suggesting that the Cu^[I] species could activate molecular oxygen to form a reactive oxygen species in situ. Interestingly, the isomeric copper complexes showed different site-specificities in the oxidation of an oligodeoxynucleotide having single-strand–double-helix junctions in the sequence. The trans isomer Cu^[I]_[2]trans-1 induced oxidation at the consecutive deoxyguanosine site (GG) near the junction; on the other hand, such site-specificity was not observed in the case of Cu^[I]_[2]cis-1.
Rights: © 2013 Elsevier B.V.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/179479
DOI(Published Version): 10.1016/j.ica.2013.06.037
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