このアイテムのアクセス数: 116

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
j.jbiosc.2013.05.023.pdf407.8 kBAdobe PDF見る/開く
タイトル: Characterization of a trifunctional fatty acid desaturase from oleaginous filamentous fungus Mortierella alpina 1S-4 using a yeast expression system.
著者: Kikukawa, Hiroshi
Sakuradani, Eiji
Kishino, Shigenobu  kyouindb  KAKEN_id
Park, Si-Bum
Ando, Akinori  kyouindb  KAKEN_id
Shima, Jun
Ochiai, Misa
Shimizu, Sakayu
Ogawa, Jun  kyouindb  KAKEN_id
著者名の別形: 菊川, 寛史
櫻谷, 英治
キーワード: Mortierella alpina
Polyunsaturated fatty acid
Trifunctional fatty acid desaturase
Hexadecadienoic acid
Hexadecatrienoic acid
発行日: Dec-2013
出版者: Elsevier B.V.
誌名: Journal of bioscience and bioengineering
巻: 116
号: 6
開始ページ: 672
終了ページ: 676
抄録: A ω3-fatty acid desaturase gene (maw3) which is involved in biosynthesis of n-3 polyunsaturated fatty acids (PUFAs) was previously isolated from Mortierella alpina 1S-4. In this report, we investigated the products of MAW3 catalyzing reaction with endogenous and exogenous fatty acids in the yeast transformant. Two unusual fatty acids de novo synthesized in the yeast transformant expressing maw3 gene were identified as n-4 hexadecadienoic acid (16:2(9cis, 12cis)) and n-1 hexadecatrienoic acid (16:3(9cis, 12cis, 15)) by GC-MS and (1)H NMR analyses. In addition to the desaturation activity at the ω3-position for 18- and 20-carbon PUFAs, MAW3 in the yeast transformant inserted a double bond at Δ12-position of endogenous palmitoleic acid (16:1(9cis)) and further at Δ15-position of the resulting 16:2(9cis, 12cis) to result in the formation of 16:3(9cis, 12cis, 15) leading to a bifunctional Δ12/Δ15-desaturase for 16-carbon fatty acids. Moreover, we evaluated the activity of MAW3 in the yeast transformant under different temperatures. The MAW3 did not have desaturation activities in M. alpina 1S-4 at 28°C but it had in the yeast transformant for various fatty acids. The MAW3 was demonstrated to be a trifunctional Δ12/Δ15/ω3-desaturase, exhibiting Δ12-desaturation for 16:1(9cis), Δ15-desaturation for 16- and 18-carbon fatty acids that had a preexisting cis-double bond at Δ12 position, and ω3-desaturation for 20-carbon fatty acids having that at Δ14-position. It is the first report that the fatty acid desaturase (MAW3) is shown to have Δ12- and Δ15-desaturation activities for a 16-carbon fatty acid, in addition to its major function, ω3-desaturation activity.
著作権等: © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/179615
DOI(出版社版): 10.1016/j.jbiosc.2013.05.023
PubMed ID: 23790547
出現コレクション:学術雑誌掲載論文等

アイテムの詳細レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。