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Title: Antibody-based analysis reveals "filamentous vs. non-filamentous" and "cytoplasmic vs. nuclear" crosstalk of cytoskeletal proteins.
Authors: Kumeta, Masahiro  kyouindb  KAKEN_id
Hirai, Yuya  KAKEN_id
Yoshimura, Shige H  kyouindb  KAKEN_id
Horigome, Tsuneyoshi
Takeyasu, Kunio
Author's alias: 粂田, 昌宏
Keywords: Cytoskeleton
Monoclonal antibody
Nuclear matrix
Nuclear scaffold
Nuclear transport
Issue Date: 10-Dec-2013
Publisher: Elsevier Inc.
Journal title: Experimental cell research
Volume: 319
Issue: 20
Start page: 3226
End page: 3237
Abstract: To uncover the molecular composition and dynamics of the functional scaffold for the nucleus, three fractions of biochemically-stable nuclear protein complexes were extracted and used as immunogens to produce a variety of monoclonal antibodies. Many helix-based cytoskeletal proteins were identified as antigens, suggesting their dynamic contribution to nuclear architecture and function. Interestingly, sets of antibodies distinguished distinct subcellular localization of a single isoform of certain cytoskeletal proteins; distinct molecular forms of keratin and actinin were found in the nucleus. Their nuclear shuttling properties were verified by the apparent nuclear accumulations under inhibition of CRM1-dependent nuclear export. Nuclear keratins do not take an obvious filamentous structure, as was revealed by non-filamentous cytoplasmic keratin-specific monoclonal antibody. These results suggest the distinct roles of the helix-based cytoskeletal proteins in the nucleus.
Rights: © 2013 Elsevier Inc.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1016/j.yexcr.2013.07.021
PubMed ID: 23911988
Appears in Collections:Journal Articles

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