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j.jconrel.2013.10.024.pdf | 457.73 kB | Adobe PDF | 見る/開く |
タイトル: | Transplantation of insulin-secreting multicellular spheroids for the treatment of type 1 diabetes in mice. |
著者: | Kusamori, Kosuke Nishikawa, Makiya Mizuno, Narumi Nishikawa, Tomoko Masuzawa, Akira Shimizu, Kazunori Konishi, Satoshi Takahashi, Yuki https://orcid.org/0000-0002-8772-2772 (unconfirmed) Takakura, Yoshinobu https://orcid.org/0000-0002-7359-2647 (unconfirmed) |
著者名の別形: | 西川, 元也 |
キーワード: | Allogeneic cell Cell viability Diabetes Multicellular spheroid Silicone elastomer Transplantation |
発行日: | 10-Jan-2014 |
出版者: | Elsevier BV |
誌名: | Journal of controlled release |
巻: | 173 |
開始ページ: | 119 |
終了ページ: | 124 |
抄録: | The efficacy of cell-based therapy depends on the function and survival of transplanted cells, which have been suggested to be enhanced by spheroid formation. However, few attempts at spheroid generation from insulin-secreting cells, which may be used to treat type 1 diabetes, have been reported. We therefore developed spheroids from the mouse insulinoma cell line NIT-1 by using polydimethylsiloxane (PDMS)-based microwells with a coating of poly(N-isopropylacrylamide) (PNIPAAm). The prepared NIT-1 spheroids or dissociated NIT-1 cells were transplanted into the subrenal capsule in streptozotocin-induced diabetic mice. NIT-1 spheroids prepared using the PNIPAAm-coated PDMS-based microwells had a uniformly sized spherical structure with a diameter of 200-300μm. The PNIPAAm coating increased cell survival in the spheroids and the recovery of the spheroids from the microwells. In diabetic mice, the transplanted NIT-1 spheroids reduced blood glucose levels to normal values faster than dissociated NIT-1 cells did. Additionally, survival was higher among NIT-1 cells in spheroids than among dissociated NIT-1 cells 24h after transplantation. These results indicate that insulin-secreting NIT-1 spheroids prepared using PNIPAAm-coated PDMS-based microwells are more effective for the treatment of type 1 diabetes than dissociated cells in suspension. |
著作権等: | © 2013 Elsevier B.V. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/179777 |
DOI(出版社版): | 10.1016/j.jconrel.2013.10.024 |
PubMed ID: | 24184345 |
出現コレクション: | 学術雑誌掲載論文等 |
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