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Title: Transplantation of insulin-secreting multicellular spheroids for the treatment of type 1 diabetes in mice.
Authors: Kusamori, Kosuke
Nishikawa, Makiya  kyouindb  KAKEN_id
Mizuno, Narumi
Nishikawa, Tomoko
Masuzawa, Akira
Shimizu, Kazunori
Konishi, Satoshi
Takahashi, Yuki  kyouindb  KAKEN_id
Takakura, Yoshinobu  kyouindb  KAKEN_id
Author's alias: 西川, 元也
Keywords: Allogeneic cell
Cell viability
Multicellular spheroid
Silicone elastomer
Issue Date: 10-Jan-2014
Publisher: Elsevier B.V.
Journal title: Journal of controlled release
Volume: 173
Start page: 119
End page: 124
Abstract: The efficacy of cell-based therapy depends on the function and survival of transplanted cells, which have been suggested to be enhanced by spheroid formation. However, few attempts at spheroid generation from insulin-secreting cells, which may be used to treat type 1 diabetes, have been reported. We therefore developed spheroids from the mouse insulinoma cell line NIT-1 by using polydimethylsiloxane (PDMS)-based microwells with a coating of poly(N-isopropylacrylamide) (PNIPAAm). The prepared NIT-1 spheroids or dissociated NIT-1 cells were transplanted into the subrenal capsule in streptozotocin-induced diabetic mice. NIT-1 spheroids prepared using the PNIPAAm-coated PDMS-based microwells had a uniformly sized spherical structure with a diameter of 200-300μm. The PNIPAAm coating increased cell survival in the spheroids and the recovery of the spheroids from the microwells. In diabetic mice, the transplanted NIT-1 spheroids reduced blood glucose levels to normal values faster than dissociated NIT-1 cells did. Additionally, survival was higher among NIT-1 cells in spheroids than among dissociated NIT-1 cells 24h after transplantation. These results indicate that insulin-secreting NIT-1 spheroids prepared using PNIPAAm-coated PDMS-based microwells are more effective for the treatment of type 1 diabetes than dissociated cells in suspension.
Rights: © 2013 Elsevier B.V.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1016/j.jconrel.2013.10.024
PubMed ID: 24184345
Appears in Collections:Journal Articles

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