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Title: A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
Authors: Nakagawa, Masato  kyouindb  KAKEN_id  orcid (unconfirmed)
Taniguchi, Yukimasa
Senda, Sho
Takizawa, Nanako
Ichisaka, Tomoko
Asano, Kanako
Morizane, Asuka  kyouindb  KAKEN_id  orcid (unconfirmed)
Doi, Daisuke  kyouindb  KAKEN_id  orcid (unconfirmed)
Takahashi, Jun  kyouindb  KAKEN_id
Nishizawa, Masatoshi
Yoshida, Yoshinori  kyouindb  KAKEN_id  orcid (unconfirmed)
Toyoda, Taro  kyouindb  KAKEN_id  orcid (unconfirmed)
Osafune, Kenji  kyouindb  KAKEN_id
Sekiguchi, Kiyotoshi
Yamanaka, Shinya  kyouindb  KAKEN_id
Author's alias: 中川, 誠人
Keywords: Embryonic stem cells
Induced pluripotent stem cells
Issue Date: 8-Jan-2014
Publisher: Nature Publishing Group
Journal title: Scientific Reports
Volume: 4
Thesis number: 3594
Abstract: In order to apply human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to regenerative medicine, the cells should be produced under restricted conditions conforming to GMP guidelines. Since the conventional culture system has some issues that need to be addressed to achieve this goal, we developed a novel culture system. We found that recombinant laminin-511 E8 fragments are useful matrices for maintaining hESCs and hiPSCs when used in combination with a completely xeno-free (Xf) medium, StemFit™. Using this system, hESCs and hiPSCs can be easily and stably passaged by dissociating the cells into single cells for long periods, without any karyotype abnormalities. Human iPSCs could be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood cells, and they possessed differentiation abilities. These results indicate that hiPSCs can be generated and maintained under this novel Ff and Xf culture system.
Description: 細胞移植に適した新しいヒトiPS細胞の樹立・維持培養法を確立. 京都大学プレスリリース. 2014-01-08.
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
DOI(Published Version): 10.1038/srep03594
PubMed ID: 24399248
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