ダウンロード数: 319
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
srep03843.pdf | 1.62 MB | Adobe PDF | 見る/開く |
タイトル: | Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts. |
著者: | Pandian, Ganesh N Taniguchi, Junichi Junetha, Syed Sato, Shinsuke Han, Le Saha, Abhijit Anandhakumar, Chandran Bando, Toshikazu Nagase, Hiroki Vaijayanthi, Thangavel Taylor, Rhys D Sugiyama, Hiroshi https://orcid.org/0000-0001-8923-5946 (unconfirmed) |
著者名の別形: | 杉山, 弘 |
キーワード: | DNA Chemical tools |
発行日: | 24-Jan-2014 |
出版者: | Nature Publishing Group |
誌名: | Scientific reports |
巻: | 4 |
論文番号: | 3843 |
抄録: | The influential role of the epigenome in orchestrating genome-wide transcriptional activation instigates the demand for the artificial genetic switches with distinct DNA sequence recognition. Recently, we developed a novel class of epigenetically active small molecules called SAHA-PIPs by conjugating selective DNA binding pyrrole-imidazole polyamides (PIPs) with the histone deacetylase inhibitor SAHA. Screening studies revealed that certain SAHA-PIPs trigger targeted transcriptional activation of pluripotency and germ cell genes in mouse and human fibroblasts, respectively. Through microarray studies and functional analysis, here we demonstrate for the first time the remarkable ability of thirty-two different SAHA-PIPs to trigger the transcriptional activation of exclusive clusters of genes and noncoding RNAs. QRT-PCR validated the microarray data, and some SAHA-PIPs activated therapeutically significant genes like KSR2. Based on the aforementioned results, we propose the potential use of SAHA-PIPs as reagents capable of targeted transcriptional activation. |
記述: | 人工スイッチを使った遺伝子コントロールに成功 -治療に役立つ可能性も- 京都大学プレスリリース. 2014-01-24. |
著作権等: | This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
URI: | http://hdl.handle.net/2433/180545 |
DOI(出版社版): | 10.1038/srep03843 |
PubMed ID: | 24457603 |
関連リンク: | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2013_1/140124_1.htm |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。