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Title: Characterization of the IL-15 niche in primary and secondary lymphoid organs in vivo.
Authors: Cui, Guangwei  kyouindb  KAKEN_id
Hara, Takahiro  kyouindb  KAKEN_id
Simmons, Szandor
Wagatsuma, Keisuke
Abe, Akifumi
Miyachi, Hitoshi
Kitano, Satsuki
Ishii, Masaru
Tani-Ichi, Shizue
Ikuta, Koichi  kyouindb  KAKEN_id
Author's alias: 生田, 宏一
Issue Date: 21-Jan-2014
Publisher: National Academy of Sciences
Journal title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 111
Issue: 5
Start page: 1915
End page: 1920
Abstract: IL-15 is a cytokine critical for development, maintenance, and response of T cells, natural killer (NK) cells, NK T cells, and dendritic cells. However, the identity and distribution of IL-15-expressing cells in lymphoid organs are not well understood. To address these questions, we established and analyzed IL-15-CFP knock-in mice. We found that IL-15 was highly expressed in thymic medulla, and medullary thymic epithelial cells with high MHC class II expression were the major source of IL-15. In bone marrow, IL-15 was detected primarily in VCAM-1(+)PDGFRβ(+)CD31(-)Sca-1(-) stromal cells, which corresponded to previously described CXCL12-abundant reticular cells. In lymph nodes, IL-15-expressing cells were mainly distributed in the T-cell zone and medulla. IL-15 was expressed in some fibroblastic reticular cells and gp38(-)CD31(-) double-negative stromal cells in the T-cell zone. Blood endothelial cells, including all high endothelial venules, also expressed high IL-15 levels in lymph nodes, whereas lymphatic endothelial cells (LECs) lacked IL-15 expression. In spleen, IL-15 was expressed in VCAM-1(+) stromal cells, where its expression increased as mice aged. Finally, IL-15 expression in blood and LECs of peripheral lymphoid organs significantly increased in LPS-induced inflammation. Overall, we have identified and characterized several IL-15-expressing cells in primary and secondary lymphoid organs, providing a unique perspective of IL-15 niche in immune microenvironment. This study also suggests that some stromal cells express IL-7 and IL-15 differentially and suggests a way to functionally classify different stromal cell subsets.
Description: サイトカインIL-15を産生する細胞の可視化に成功 -免疫系の微小環境の解明に期待-. 京都大学プレスリリース. 2014-01-21.
Rights: © 2014 National Academy of Sciences.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1073/pnas.1318281111
PubMed ID: 24449915
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