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ten.tea.2007.0297.pdf | 892.14 kB | Adobe PDF | 見る/開く |
タイトル: | Adipogenesis induced by human adipose tissue-derived stem cells. |
著者: | Tsuji, Wakako Inamoto, Takashi Yamashiro, Hiroyasu Ueno, Takayuki Kato, Hironori Kimura, Yu https://orcid.org/0000-0001-5318-9255 (unconfirmed) Tabata, Yasuhiko Toi, Masakazu https://orcid.org/0000-0003-1488-9958 (unconfirmed) |
著者名の別形: | 辻, 和香子 |
発行日: | 20-Jul-2009 |
出版者: | Mary Ann Liebert |
誌名: | Tissue engineering. Part A |
巻: | 15 |
号: | 1 |
開始ページ: | 83 |
終了ページ: | 93 |
抄録: | Adipose tissue-derived stem cells (ASCs), including preadipocytes, may play an important role in de novo adipogenesis and are expected to be a useful external source of cells for adipose tissue engineering. In this study, we examined in vivo adipogenesis up to 24 weeks after implantation, induced by human ASCs that were isolated from adipose tissues and expanded in vitro. ASCs proliferated in vitro in the presence of basic fibroblast growth factor (bFGF), and the number of cells increased by more than 1000-fold at the fourth passage. The ability to differentiate into mature adipocytes was maintained up to the third passage. We incorporated designated numbers of third-passage-expanded cells into a type I collagen scaffold and implanted them into the back of nude mice with or without controlled-release bFGF. After the implantation of 2 x 10(6) ASCs with controlled-release bFGF, the greatest cross-sectional surface area of adipose tissue in the scaffold was 1.19 mm(2) at 12 weeks and 2.14 mm(2) at 24 weeks. About 2 x 10(6) ASCs with controlled-release bFGF was the best condition for total adipogenesis. Immunohistochemical analysis with antihuman vimentin antibody showed that the area of human-origin adipose tissue was maximum in the group with 8 x 10(6) ASCs incorporated in a scaffold at both 12 and 24 weeks. The amount of human-origin adipose tissue increased in all groups with implanted ASCs from 12 to 24 weeks. Only trace of human-origin adipose tissue was observed in other groups implanted ASCs. Our results show that human ASCs not only function as progenitor cells for in vivo adipogenesis, but also induce de novo adipogenesis for long period. |
著作権等: | This is a copy of an article published in the "Tissue Eng Part A." © 2009 Mary Ann Liebert, Inc.; "Adipogenesis induced by human adipose derived stem cells" is available online at: http://online.liebertpub.com. |
URI: | http://hdl.handle.net/2433/181188 |
DOI(出版社版): | 10.1089/ten.tea.2007.0297 |
PubMed ID: | 18759663 |
出現コレクション: | 学術雑誌掲載論文等 |
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