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タイトル: Preparation of gelatin hydrogels incorporating small interfering RNA for the controlled release.
著者: Saito, Takashi
Tabata, Yasuhiko  kyouindb  KAKEN_id
著者名の別形: 田畑, 泰彦
キーワード: Gelatin hydrogel
controlled release
siRNA
gene delivery
発行日: Dec-2012
出版者: Informa Healthcare
誌名: Journal of drug targeting
巻: 20
号: 10
開始ページ: 864
終了ページ: 872
抄録: The objective of this study is to design biodegradable hydrogels of gelatin for the controlled release of bioactive small interfering RNA (siRNA). Gelatin was cationized by chemically introducing ethylene diamine into the carboxyl groups in different conditions to obtain cationized gelatins (CGs). The CG was mixed with siRNA at various mixing ratios of amino groups of gelatin to the phosphate groups of siRNA in aqueous solution to form the siRNA-CGnano-complex. Gelatin together with the complex of siRNA and CG was chemically crosslinked to prepare the gelatin hydrogel incorporating the complex. The hydrogel incorporating the siRNA-CG complex was hardly degraded in phosphate-buffered saline solution (PBS) at 37°C and did not release the siRNA complex after initial siRNA release. When placed in PBS containing collagenase, the hydrogel was enzymatically degraded to release the siRNA complex. In this release system, the siRNA was released as a result of hydrogel degradation. The siRNA released showed an activity to specifically suppress the expression of the corresponding gene. The hydrogel is promising to release siRNA and also elicit biological activity for a longer time.
著作権等: © 2012 Informa Plc.
This is not the published version. Please cite only the published version.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/184918
DOI(出版社版): 10.3109/1061186X.2012.725170
PubMed ID: 23009281
出現コレクション:学術雑誌掲載論文等

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