|Title:||A chemical probe that labels human pluripotent stem cells.|
Inoue, Haruhisa https://orcid.org/0000-0003-4736-9537 (unconfirmed)
Suemori, Hirofumi https://orcid.org/0000-0002-3565-3111 (unconfirmed)
Eto, Koji https://orcid.org/0000-0002-5863-7122 (unconfirmed)
Ueda, Kazumitsu https://orcid.org/0000-0003-2980-6078 (unconfirmed)
Uesugi, Motonari https://orcid.org/0000-0002-8515-445X (unconfirmed)
|Author's alias:||中辻, 憲夫|
|Journal title:||Cell reports|
|Abstract:||A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.|
|Rights:||© 2014 The Authors. Published by Elsevier Inc.|
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
|Appears in Collections:||Journal Articles|
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