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ファイル | 記述 | サイズ | フォーマット | |
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j.celrep.2014.02.006.pdf | 2.26 MB | Adobe PDF | 見る/開く |
タイトル: | A chemical probe that labels human pluripotent stem cells. |
著者: | Hirata, Nao Nakagawa, Masato https://orcid.org/0000-0003-3067-7322 (unconfirmed) Fujibayashi, Yuto Yamauchi, Kaori Murata, Asako Minami, Itsunari Tomioka, Maiko Kondo, Takayuki Kuo, Ting-Fang Endo, Hiroshi Inoue, Haruhisa https://orcid.org/0000-0003-4736-9537 (unconfirmed) Sato, Shin-Ichi Ando, Shin Kawazoe, Yoshinori Aiba, Kazuhiro Nagata, Koh Kawase, Eihachiro https://orcid.org/0000-0002-8285-5971 (unconfirmed) Chang, Young-Tae Suemori, Hirofumi https://orcid.org/0000-0002-3565-3111 (unconfirmed) Eto, Koji https://orcid.org/0000-0002-5863-7122 (unconfirmed) Nakauchi, Hiromitsu Yamanaka, Shinya Nakatsuji, Norio Ueda, Kazumitsu https://orcid.org/0000-0003-2980-6078 (unconfirmed) Uesugi, Motonari https://orcid.org/0000-0002-8515-445X (unconfirmed) |
著者名の別形: | 中辻, 憲夫 |
発行日: | 27-Mar-2014 |
出版者: | Elsevier B.V. |
誌名: | Cell reports |
巻: | 6 |
号: | 6 |
開始ページ: | 1165 |
終了ページ: | 1174 |
抄録: | A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents. |
著作権等: | © 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). |
URI: | http://hdl.handle.net/2433/187095 |
DOI(出版社版): | 10.1016/j.celrep.2014.02.006 |
PubMed ID: | 24613351 |
出現コレクション: | 学術雑誌掲載論文等 |
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