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Title: A chemical probe that labels human pluripotent stem cells.
Authors: Hirata, Nao
Nakagawa, Masato
Fujibayashi, Yuto
Yamauchi, Kaori
Murata, Asako
Minami, Itsunari  kyouindb  KAKEN_id
Tomioka, Maiko
Kondo, Takayuki
Kuo, Ting-Fang
Endo, Hiroshi
Inoue, Haruhisa  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-4736-9537 (unconfirmed)
Sato, Shin-Ichi
Ando, Shin
Kawazoe, Yoshinori
Aiba, Kazuhiro  KAKEN_id
Nagata, Koh
Kawase, Eihachiro  kyouindb  KAKEN_id
Chang, Young-Tae
Suemori, Hirofumi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3565-3111 (unconfirmed)
Eto, Koji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-5863-7122 (unconfirmed)
Nakauchi, Hiromitsu
Yamanaka, Shinya  kyouindb  KAKEN_id
Nakatsuji, Norio  kyouindb  KAKEN_id
Ueda, Kazumitsu  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-2980-6078 (unconfirmed)
Uesugi, Motonari  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-8515-445X (unconfirmed)
Author's alias: 中辻, 憲夫
Issue Date: 27-Mar-2014
Publisher: Elsevier B.V.
Journal title: Cell reports
Volume: 6
Issue: 6
Start page: 1165
End page: 1174
Abstract: A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.
Rights: © 2014 The Authors. Published by Elsevier Inc.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
URI: http://hdl.handle.net/2433/187095
DOI(Published Version): 10.1016/j.celrep.2014.02.006
PubMed ID: 24613351
Appears in Collections:Journal Articles

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