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Title: Two types of alcohol dehydrogenase from Perilla can form citral and perillaldehyde.
Authors: Sato-Masumoto, Naoko
Ito, Michiho
Author's alias: 佐藤(増本) , 直子
伊藤, 美千穂
Keywords: Perilla
Labiatae
Aldo–keto reductase
Geraniol dehydrogenase
Geraniol
Citral
Sequential enzymatic reaction
Biosynthetic pathway
Essential oil
Issue Date: Aug-2014
Publisher: Elsevier Ltd.
Journal title: Phytochemistry
Volume: 104
Start page: 12
End page: 20
Abstract: Studies on the biosynthesis of oil compounds in Perilla will help in understanding regulatory systems of secondary metabolites and in elucidating reaction mechanisms for natural product synthesis. In this study, two types of alcohol dehydrogenases, an aldo-keto reductase (AKR) and a geraniol dehydrogenase (GeDH), which are thought to participate in the biosynthesis of perilla essential oil components, such as citral and perillaldehyde, were isolated from three pure lines of perilla. These enzymes shared high amino acid sequence identity within the genus Perilla, and were expressed regardless of oil type. The overall reaction from geranyl diphosphate to citral was performed in vitro using geraniol synthase and GeDH to form a large proportion of citral and relatively little geraniol as reaction products. The biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil, was established in this study.
Rights: © 2014 Elsevier Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/187787
DOI(Published Version): 10.1016/j.phytochem.2014.04.019
PubMed ID: 24864017
Appears in Collections:Journal Articles

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