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ファイル | 記述 | サイズ | フォーマット | |
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j.phytochem.2014.04.019.pdf | 2.86 MB | Adobe PDF | 見る/開く |
タイトル: | Two types of alcohol dehydrogenase from Perilla can form citral and perillaldehyde. |
著者: | Sato-Masumoto, Naoko Ito, Michiho https://orcid.org/0000-0001-9821-0628 (unconfirmed) |
著者名の別形: | 佐藤(増本) , 直子 伊藤, 美千穂 |
キーワード: | Perilla Labiatae Aldo–keto reductase Geraniol dehydrogenase Geraniol Citral Sequential enzymatic reaction Biosynthetic pathway Essential oil |
発行日: | Aug-2014 |
出版者: | Elsevier Ltd. |
誌名: | Phytochemistry |
巻: | 104 |
開始ページ: | 12 |
終了ページ: | 20 |
抄録: | Studies on the biosynthesis of oil compounds in Perilla will help in understanding regulatory systems of secondary metabolites and in elucidating reaction mechanisms for natural product synthesis. In this study, two types of alcohol dehydrogenases, an aldo-keto reductase (AKR) and a geraniol dehydrogenase (GeDH), which are thought to participate in the biosynthesis of perilla essential oil components, such as citral and perillaldehyde, were isolated from three pure lines of perilla. These enzymes shared high amino acid sequence identity within the genus Perilla, and were expressed regardless of oil type. The overall reaction from geranyl diphosphate to citral was performed in vitro using geraniol synthase and GeDH to form a large proportion of citral and relatively little geraniol as reaction products. The biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil, was established in this study. |
著作権等: | © 2014 Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/187787 |
DOI(出版社版): | 10.1016/j.phytochem.2014.04.019 |
PubMed ID: | 24864017 |
出現コレクション: | 学術雑誌掲載論文等 |
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