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j.nano.2013.11.017.pdf1 MBAdobe PDF見る/開く
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dc.contributor.authorUno, Shotaja
dc.contributor.authorNishikawa, Makiyaja
dc.contributor.authorMohri, Kohtaja
dc.contributor.authorUmeki, Yukaja
dc.contributor.authorMatsuzaki, Noriyukija
dc.contributor.authorTakahashi, Yukija
dc.contributor.authorFujita, Haruyukija
dc.contributor.authorKadowaki, Norimitsuja
dc.contributor.authorTakakura, Yoshinobuja
dc.contributor.alternative西川, 元也ja
dc.date.accessioned2014-06-06T05:36:12Z-
dc.date.available2014-06-06T05:36:12Z-
dc.date.issued2014-05ja
dc.identifier.issn1549-9634ja
dc.identifier.urihttp://hdl.handle.net/2433/187794-
dc.description.abstractInvestigation of mouse macrophage-like RAW264.7 cells showed that the immunostimulatory activity of CpG DNA is increased by formation of polypod-like structured DNA (polypodna), an assembly consisting of three or more oligodeoxynucleotides. To apply CpG polypodna to immunotherapy, its activity was examined in murine dendritic DC2.4 cells, splenic macrophages, and bone marrow-derived dendritic cells (BMDCs). In all cell types, increasing the pod number increased the cellular uptake of DNA and cytokine release. No significant release of cytokines was observed in macrophages lacking Toll-like receptor 9. Similar results were obtained after intradermal injection of polypodna. The polypodna preparations produced significantly higher amounts of interferon α in human peripheral blood mononuclear cells (PBMCs) compared with single-stranded DNA. The conditioned medium of hexapodna-treated human PBMCs effectively inhibited the activity of a hepatitis C virus subgenomic replicon reporter system. These results indicate that polypodna preparations are useful as an immunostimulator.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherElsevier Inc.ja
dc.rights© 2014 Elsevier Inc.ja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectCpG DNAja
dc.subjectToll-like receptor 9ja
dc.subjectDendritic cellja
dc.subjectPeripheral blood mononuclear cellja
dc.titleEfficient delivery of immunostimulatory DNA to mouse and human immune cells through the construction of polypod-like structured DNA.ja
dc.type.niitypeJournal Articleja
dc.identifier.jtitleNanomedicineja
dc.identifier.volume10ja
dc.identifier.issue4ja
dc.identifier.spage765ja
dc.identifier.epage774ja
dc.relation.doi10.1016/j.nano.2013.11.017ja
dc.textversionauthorja
dc.identifier.pmid24333587ja
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